LOCUS       sequence-281579-        2456 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1693
FEATURES             Location/Qualifiers
     source          1..2456
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagggcgc gatggcagtg cgggtgacgg ccgctcatac ggcagaagcc
       61 caggccgaag ccgggcggga agcgggcgag ggtgcagttg cggcggtggc ggcggccttg
      121 gcccccagcg gcttcctcgg cctcccggcg cccttcagcg aggaagatga ggacgatgtg
      181 cacagatgcg gccgctgcca ggcagagttc accgccttgg aggattttgt tcagcacaag
      241 attcagaagg cctgccagcg ggcccctccg gaggccctgc ctgccacccc tgccaccaca
      301 gcgttgctgg gccaggaggt ggtgccggca gcaccaggcc cagaggagcc catcactgtg
      361 gcccacatcg tggtggaggc ggcctctctg gcagcagaca tcagccacgc atctgacctt
      421 gttggtggtg ggcacatcaa agaggtcatc gtggctgctg aggcggagct gggagacggt
      481 gagatggccg aggccccggg cagcccccgc cagcaggggc tggggctcgc aggggagggt
      541 gagcaggccc aggtgaagct actggtgaac aaggatggcc gctatgtgtg tgcgctgtgc
      601 cacaagacct tcaagacggg cagcatcctc aaggcccaca tggtcactca cagcagccgc
      661 aaggaccacg agtgcaagct ctgtggggcc tccttccgca ccaagggctc actcatccgg
      721 caccaccggc ggcacacgga tgagcgcccc tacaagtgct ccaagtgtgg aaagagcttc
      781 cgggagtcgg gtgcactgac ccggcacctc aagtctctca ccccctgcac agagaaaatc
      841 cgcttcagtg tgagcaagga cgtggttgtc agcaaagagg acgcacgtgc aggttctgga
      901 gctggagctg ccggcttggg gacagccaca tcatcggtga caggcgagcc tatagagact
      961 tcacccgtga ttcacctggt gacagatgcc aagggcaccg tcatccacga agtccacgtc
     1021 cagatgcagg agctgtccct gggcatgaaa gccctggccc cagagccccc cgtctcccag
     1081 gagctcccct gctccagcga gggcagccgt gagaacctgc tgcaccaggc catgcagaac
     1141 tccggcatcg tccttgagcg cgctgctggg gaggagggtg ccctggagcc agctcctgct
     1201 gccgggtcca gtccccagcc cctggcagtg gcagccccgc agctgccggt actggaagtg
     1261 cagccgctgg agacacaggt ggccagcgag gcctcagcgg tgcccaggac ccacccatgt
     1321 cctcagtgca gtgagacctt cccgacagca gccaccctgg aggcccacaa gaggggccac
     1381 accgggccga ggccgttcgc ctgcgcgcag tgtggcaagg ccttccccaa ggcctacctg
     1441 ctcaagaagc accaggaggt gcacgtgcgt gagcgccgct tccgctgtgg cgactgcggg
     1501 aagctctaca agaccattgc ccatgtgcgt ggccaccggc gcgtccactc agacgagcgg
     1561 ccctaccctt gtcccaagtg tggcaagcgc tacaagacta agaacgcaca gcaggtgcac
     1621 ttcaggacac acctggagga gaagccgcac gtgtgccagt tctgcagccg tggcttccga
     1681 gagaagggct cactggtgcg gcacgtgcga caccacacag gcgagaagcc gttcaagtgc
     1741 tacaagtgcg gccgtggctt cgccgagcac ggcacgctga accggcacct gcgcaccaaa
     1801 gggggctgcc tgctggaggt ggaggagttg ctggtgtctg aggacagccc cgcggcagcc
     1861 accaccgtcc tcacggaaga cccgcacaca gtgttggtgg agttctcgtc cgtggtagct
     1921 gacacccagg agtatatcat cgaggccact gcggacgatg cggagaccag tgaggccacg
     1981 gagatcatcg agggcaccca gacagaggtg gacagccaca tcatgaaggt ggtgcagcag
     2041 atcgtgcacc aggctagcgc cggccaccag atcatcgtgc agaacgtcac catggacgag
     2101 gagacggcgc tgggcccaga ggcggctgcc gccgacacca tcaccatcgc cacccccgag
     2161 agcctgacag agcaggtggc catgacgctg gcctcggcca tcagcgaggg cactgtgctt
     2221 gccgcccggg cagggacaag tggcactgaa caggccactg tgaccatggt gtcatcagag
     2281 gacatcgaga tcctggagca tgcaggcgag ctggtcatcg cctcgccgga gggccagctg
     2341 gaggtgcaga cggtcatcgt ctagtaatga actagtccgt aacttgaaag tatttcgatt
     2401 tcttggcttt atatatcttg tggaaaggac gagtgagtgg tggaagatat ggctca
//
