LOCUS       sequence-281582-        1925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1694
FEATURES             Location/Qualifiers
     source          1..1925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagggcgc gatggcagtg cgggtgacgg ccgctcatac ggcagaagcc
       61 caggccgaag ccgggcggga agcgggcgag ggtgcagttg cggcggtggc ggcggccttg
      121 gcccccagcg gcttcctcgg cctcccggcg cccttcagcg aggaagatga ggacgatgtg
      181 cacagatgcg gccgctgcca ggcagagttc accgccttgg aggattttgt tcagcacaag
      241 attcagaagg cctgccagcg ggcccctccg gaggccctgc ctgccacccc tgccaccaca
      301 gcgttgctgg gccaggaggt ggtgccggca gcaccaggcc cagaggagcc catcactgtg
      361 gcccacatcg tggtggaggc ggcctctctg gcagcagaca tcagccacgc atctgacctt
      421 gttggtggtg ggcacatcaa agaggtcatc gtggctgctg aggcggagct gggagacggt
      481 gagatggccg aggccccggg cagcccccgc cagcaggggc tggggctcgc aggggagggt
      541 gagcaggccc aggtgaagct actggtgaac aaggatggcc gctatgtgtg tgcgctgtgc
      601 cacaagacct tcaagacggg cagcatcctc aaggcccaca tggtcactca cagcagccgc
      661 aaggaccacg agtgcaagct ctgtggggcc tccttccgca ccaagggctc actcatccgg
      721 caccaccggc ggcacacgga tgagcgcccc tacaagtgct ccaagtgtgg aaagagcttc
      781 cgggagtcgg gtgcactgac ccggcacctc aagtctctca ccccctgcac agagaaaatc
      841 cgcttcagtg tgagcaagga cgtggttgtc agcaaagagg acgcacgtgc aggttctgga
      901 gctggagctg ccggcttggg gacagccaca tcatcggtga caggcgagcc tatagagact
      961 tcacccgtga ttcacctggt gacagatgcc aagggcaccg tcatccacga agtccacgtc
     1021 cagatgcagg agctgtccct gggcatgaaa gccctggccc cagagccccc cgtctcccag
     1081 gagctcccct gctccagcga gggcagccgt gagaacctgc tgcaccagtt ctgcagccgt
     1141 ggcttccgag agaagggctc actggtgcgg cacgtgcgac accacacagg cgagaagccg
     1201 ttcaagtgct acaagtgcgg ccgtggcttc gccgagcacg gcacgctgaa ccggcacctg
     1261 cgcaccaaag ggggctgcct gctggaggtg gaggagttgc tggtgtctga ggacagcccc
     1321 gcggcagcca ccaccgtcct cacggaagac ccgcacacag tgttggtgga gttctcgtcc
     1381 gtggtagctg acacccagga gtatatcatc gaggccactg cggacgatgc ggagaccagt
     1441 gaggccacgg agatcatcga gggcacccag acagaggtgg acagccacat catgaaggtg
     1501 gtgcagcaga tcgtgcacca ggctagcgcc ggccaccaga tcatcgtgca gaacgtcacc
     1561 atggacgagg agacggcgct gggcccagag gcggctgccg ccgacaccat caccatcgcc
     1621 acccccgaga gcctgacaga gcaggtggcc atgacgctgg cctcggccat cagcgagggc
     1681 actgtgcttg ccgcccgggc agggacaagt ggcactgaac aggccactgt gaccatggtg
     1741 tcatcagagg acatcgagat cctggagcat gcaggcgagc tggtcatcgc ctcgccggag
     1801 ggccagctgg aggtgcagac ggtcatcgtc tagtaatgaa ctagtccgta acttgaaagt
     1861 atttcgattt cttggcttta tatatcttgt ggaaaggacg aatatgatgc gagcggcgca
     1921 cggtt
//
