LOCUS       sequence-281600-        1328 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1744
FEATURES             Location/Qualifiers
     source          1..1328
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgccgcc tctggccccg ctccctcccc gggacccaaa cggggccgga
       61 cccgagtgga gggagccggg ggctgtgagc ttcgcggacg tggccgtgta cttctgccgg
      121 gaggagtggg gctgcttgcg gccagcgcag agggccctgt accgggacgt gatgcgggag
      181 acctacggcc acctgagcgc tctcggaatc ggaggcaaca agccagctct catctcctgg
      241 gtggaggagg aggccgaact gtggggtccg gctgcccagg atccggaggt ggcgaaatgt
      301 cagacacaaa cggacccagc agattccaga aacaagaaaa aggaaagaca aagggaaggg
      361 acgggagccc tggagaagcc cgaccctgtg gccgccgggt ctcctgggct gaagtctccc
      421 caagccccct cggccgggcc cccttatggt tgggagcagc tgtccaaggc accgcaccgg
      481 ggacgcccct ccctctgtgc ccacccccct gtcccccgag ctgaccagcg tcatggctgc
      541 tacgtgtgcg ggaagagctt tgcctggcgc tccacactgg tggagcacgt ctacagtcac
      601 actggcgaga agcccttcca ctgcactgac tgcggcaagg gcttcggcca cgcttcctcc
      661 ctgagcaaac accgggccat ccatcgtggg gagcggcccc accgctgtct ggagtgtggc
      721 cgggccttca cgcagcgctc ggcgctgact tcgcacctgc gcgtccacac cggcgagaaa
      781 ccctatggct gcgccgactg tggccgccgc ttcagccaga gctctgccct ctaccagcac
      841 cggcgcgtgc acagcggcga gacccccttc ccctgcccgg actgtggccg cgccttcgcc
      901 tacccctcgg acctgcggcg ccacgtgcgc acccacaccg gcgagaagcc ctacccgtgc
      961 ccggactgcg ggcgctgctt ccgccagagc tcggagatgg cagcccacag gcgcacccac
     1021 agcggcgaga agccctaccc ctgcccgcag tgcggccgcc gctttggcca gaagtcagcc
     1081 gtggccaaac accagtgggt tcatcggccc ggggccgggg gccacagggg ccgggtcgcc
     1141 gggcgtctgt ctgtgaccct gacccctggc cacggagacc tggacccgcc cgtgggcttc
     1201 cagctgtacc cggagatatt ccaggagtgt gggtagtaat gaactagtcc gtaacttgaa
     1261 agtatttcga tttcttggct ttatatatct tgtggaaagg acgagatatt cctctatggt
     1321 cacctcag
//
