LOCUS       sequence-281621-        2189 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1837
FEATURES             Location/Qualifiers
     source          1..2189
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggccagac tgggaagaaa tctgagaagg gaccagtttg ttggcggaag
       61 cgtgtaaaat cagagtacat gcgactgaga cagctcaaga ggttcagacg agctgatgaa
      121 gtaaagagta tgtttagttc caatcgtcag aaaattttgg aaagaacgga aatcttaaac
      181 caagaatgga aacagcgaag gatacagcct gtgcacatcc tgacttcttg ttcggtgacc
      241 agtgacttgg attttccaac acaagtcatc ccattaaaga ctctgaatgc agttgcttca
      301 gtacccataa tgtattcttg gtctccccta cagcagaatt ttatggtgga agatgaaact
      361 gttttacata acattcctta tatgggagat gaagttttag atcaggatgg tactttcatt
      421 gaagaactaa taaaaaatta tgatgggaaa gtacacgggg atagagaatg tgggtttata
      481 aatgatgaaa tttttgtgga gttggtgaat gcccttggtc aatataatga tgatgacgat
      541 gatgatgatg gagacgatcc tgaagaaaga gaagaaaagc agaaagatct ggaggatcac
      601 cgagatgata aagaaagccg cccacctcgg aaatttcctt ctgataaaat ttttgaagcc
      661 atttcctcaa tgtttccaga taagggcaca gcagaagaac taaaggaaaa atataaagaa
      721 ctcaccgaac agcagctccc aggcgcactt cctcctgaat gtacccccaa catagatgga
      781 ccaaatgcta aatctgttca gagagagcaa agcttacact cctttcatac gcttttctgt
      841 aggcgatgtt ttaaatatga ctgcttccta catccttttc atgcaacacc caacacttat
      901 aagcggaaga acacagaaac agctctagac aacaaacctt gtggaccaca gtgttaccag
      961 catttggagg gagcaaagga gtttgctgct gctctcaccg ctgagcggat aaagacccca
     1021 ccaaaacgtc caggaggccg cagaagagga cggcttccca ataacagtag caggcccagc
     1081 acccccacca ttaatgtgct ggaatcaaag gatacagaca gtgataggga agcagggact
     1141 gaaacggggg gagagaacaa tgataaagaa gaagaagaga agaaagatga aacttcgagc
     1201 tcctctgaag caaattctcg gtgtcaaaca ccaataaaga tgaagccaaa tattgaacct
     1261 cctgagaatg tggagtggag tggtgctgaa gcctcaatgt ttagagtcct cattggcact
     1321 tactatgaca atttctgtgc cattgctagg ttaattggga ccaaaacatg tagacaggtg
     1381 tatgagttta gagtcaaaga atctagcatc atagctccag ctcccgctga ggatgtggat
     1441 actcctccaa ggaaaaagaa gaggaaacac cggttgtggg ctgcacactg cagaaagata
     1501 cagctgaaaa agggtcaaaa ccgctttccg ggatgccgct gcaaagcaca gtgcaacacc
     1561 aagcagtgcc cgtgctacct ggctgtccga gagtgtgacc ctgacctctg tcttacttgt
     1621 ggagccgctg accattggga cagtaaaaat gtgtcctgca agaactgcag tattcagcgg
     1681 ggctccaaaa agcatctatt gctggcacca tctgacgtgg caggctgggg gatttttatc
     1741 aaagatcctg tgcagaaaaa tgaattcatc tcagaatact gtggagagat tatttctcaa
     1801 gatgaagctg acagaagagg gaaagtgtat gataaataca tgtgcagctt tctgttcaac
     1861 ttgaacaatg attttgtggt ggatgcaacc cgcaagggta acaaaattcg ttttgcaaat
     1921 cattcggtaa atccaaactg ctatgcaaaa gttatgatgg ttaacggtga tcacaggata
     1981 ggtatttttg ccaagagagc catccagact ggcgaagagc tgttttttga ttacagatac
     2041 agccaggctg atgccctgaa gtatgtcggc atcgaaagag aaatggaaat cccttagtaa
     2101 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     2161 gacgatctga gcaagcaacg tgttagccg
//
