LOCUS       sequence-281630-        2093 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1877
FEATURES             Location/Qualifiers
     source          1..2093
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgtcggg ccgtggagct tcttctcgct ggttctttac tcgggaacag
       61 ctggagaaca cgccgagccg ccgctgcgga gtggaggcgg ataaagagct ctcgtgccgc
      121 cagcaggcgg ccaacctcat ccaggagatg ggacagcgtc tcaatgtctc tcagcttaca
      181 ataaacactg cgattgttta tatgcacagg ttttatatgc accattcttt caccaaattc
      241 aacaaaaata taatatcgtc tactgcatta tttttggctg caaaagtgga agaacaggct
      301 cgaaaacttg aacatgttat caaagtagca catgcttgtc ttcatcctct agagccactg
      361 ctggatacta aatgtgatgc ttaccttcaa cagactcaag aactggttat acttgaaacc
      421 ataatgctac aaactctagg ttttgagatc accattgaac acccacacac agatgtggtg
      481 aaatgtaccc agttagtaag agcaagcaag gatttggcac agacatccta tttcatggct
      541 accaacagtc tgcatcttac aaccttctgt cttcagtaca aaccaacagt gatagcatgt
      601 gtatgcattc atttggcttg caaatggtcc aattgggaga tccctgtatc aactgatgga
      661 aagcattggt gggaatatgt ggatcctaca gttactctag aattattaga tgagctaaca
      721 catgagtttc tacaaatatt ggagaaaacg cctaataggt tgaagaagat tcgaaactgg
      781 agggctaatc aggcagctag gaaaccaaaa gtagatggac aggtatcaga gacaccactt
      841 cttggttcat ctttggtcca gaattccatt ttagtagata gtgtcactgg tgtgcctaca
      901 aacccaagtt ttcagaaacc atctacatca gcattccctg cgccagtacc tctaaattca
      961 ggaaatattt ctgttcaaga cagccataca tctgataatt tgtcaatgct agcaacagga
     1021 atgccaagta cttcatacgg tttatcatca caccaggaat ggcctcaaca tcaagactca
     1081 gcaaggacag aacagctata ttcacagaaa caggagacat ctttgtctgg tagccagtac
     1141 aacatcaact tccagcaggg accttctata tcactgcatt caggattaca tcacagacct
     1201 gacaaaattt cagatcattc ttctgttaag caagaatata ctcataaagc agggagcagt
     1261 aaacaccatg ggccaatttc cactactcca ggaataattc ctcagaaaat gtctttagat
     1321 aaatatagag aaaagcgtaa actagaaact cttgatctcg atgtaaggga tcattatata
     1381 gctgcccagg tagaacagca gcacaaacaa gggcagtcac aggcagccag cagcagttct
     1441 gttacttctc ccattaaaat gaaaatacct atcgcaaata ctgaaaaata catggcagac
     1501 aaaaaggaaa agagtgggtc actgaaatta cggattccaa taccacccac tgataaaagc
     1561 gccagtaaag aagaactgaa aatgaaaata aaagtttctt cttcagaaag acacagctct
     1621 tctgatgaag gcagtgggaa gagcaaacat tcaagcccac atattagcag agaccataag
     1681 gagaagcaca aggagcatcc ttcaagccgc caccacacca gcagccacaa gcattcccac
     1741 tcgcatagtg gcagcagcag cggtggcagt aaacacagtg ccgacggaat accacccact
     1801 gttctgagga gtcctgttgg cctgagcagt gatggcattt cctctagctc cagctcttca
     1861 aggaagaggc tgcatgtcaa tgatgcatct cacaaccacc actccaaaat gagcaaaagt
     1921 tccaaaagtt caggtgggct acggacatct cagcaccctc gtgaaactgg acaagaagcc
     1981 agtggagacc aacggtccta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     2041 tggctttata tatcttgtgg aaaggacgat ctgtaattac cctgctaagt agc
//
