LOCUS       sequence-281645-        1640 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1945
FEATURES             Location/Qualifiers
     source          1..1640
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggagcc atctgttgaa tcttcaagtc caggaggttc agcaacatca
       61 gatgaccatg aatttgatcc atcagctgac atgctggttc atgattttga tgatgaacga
      121 acattagaag aggaagaaat gatggaagga gaaacaaact tcagctctga aatagaagat
      181 cttgcaaggg aaggcgacat gccaattcat gaacttctca gcctttatgg ttatggtagt
      241 actgttcgac tacctgaaga agatgaggaa gaggaagaag aggaagaaga aggtgaagat
      301 gatgaagatg ctgataatga tgacaacagt ggctgtagtg gggaaaataa agaggagaat
      361 ataaaggatt catcaggtca ggaggatgaa actcagtctt ccaatgatga tccatcacaa
      421 tctgttgctt ctcaagatgc ccaggaaata atccgcccac gtcgatgtaa atattttgat
      481 acaaatagtg aagtagaaga agaatctgaa gaagatgaag attatattcc atcagaagac
      541 tggaaaaagg agattatggt gggctccatg tttcaagcag aaattccagt tggcatttgt
      601 agatacaaag aaaatgaaaa agtatatgaa aatgatgatc agctcctgtg ggaccctgag
      661 tacttaccag aagataaagt gattatattt cttaaagatg catctagaag aacaggtgat
      721 gagaagggtg tagaagcaat tcctgaagga tctcacataa aagacaatga acaggcttta
      781 tatgaattgg ttaaatgcaa ttttgataca gaagaagcat tgagaagatt aagatttaat
      841 gtaaaagcag ctagagagga attatctgtt tggacagagg aagagtgtag aaattttgaa
      901 caagggctga aggcctatgg aaaggatttt catttgattc aggctaataa agtccgaaca
      961 aggtcagttg gtgaatgtgt agcattctat tacatgtgga aaaaatctga acgttatgat
     1021 ttctttgctc agcaaacacg atttggaaag aagaaatata atcttcatcc tggtgtaacg
     1081 gattacatgg atcgtcttct agacgaaagt gaaagtgctg catctagtcg agcaccatcc
     1141 cctcccccaa ctgcatcaaa cagtagtaac agccagtctg agaaagaaga tggcactgta
     1201 agcactgcta atcaaaatgg agtgtcatct aatggaccag gtgaaatatt aaacaaagag
     1261 gaagtaaaag ttgaagggtt acacattaat ggaccaacag gtggaaataa gaaaccactt
     1321 catgcagata tggatactaa tggttatgaa acagataacc ttaccactga cccaaaactt
     1381 gcccatatga ctgcaagaaa tgaaaatgat tttgatgaaa aaagtgagag acctgccaaa
     1441 aggcgaaggg taaacagcaa tggaaaagaa agtccaggtt cttctgaatt tttccaagaa
     1501 gcagtctcac atgggaaatt tgaagaactt gaaaacacag atgactagta atgaactagt
     1561 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgagtta
     1621 tctttaatga ggcctccatg
//
