LOCUS       sequence-281648-         683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1985
FEATURES             Location/Qualifiers
     source          1..683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcccttac tcaggggcaa ttgtcattca gcgatgtggc catagaattc
       61 tctcaggagg agtggaaatg cctggaccct gggcagaaag ctttatacag ggacgtgatg
      121 ttagagaact acaggaacct ggtctcccta ggaatctgtc ttcctgacct gagtgttacc
      181 tccatgttgg agcaaaagag agatccctgg actctgcaga gtgaagtgaa aataataaac
      241 aatccagatg gcagggagtg catcaaaggt gtgaacacag atttttcact tctggcctgg
      301 gacgcgcgcg tcttcagggt ggaagcctgg cgcgcgtccg gaggtgctga ggagccaacc
      361 ggcccaaact ttgtgggaaa tgactcccct ctgccctcgc ccctcgctct gctaccattt
      421 ccttccgttt ctgcttcgct tggcgatgca aaacgcgcga ggctcacggc agagggccga
      481 ggccacggga ctctcagggc cgggcccgcc cctcggccgc gcagtgcggc gccgcacgcc
      541 ccggacgctg tccagcgttg gccgcctgag cagaggctgc cgcggagcta gtaatgaact
      601 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
      661 ccgctacgga catacacatc ctc
//
