LOCUS       sequence-281651-         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2005
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(300..311)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 accgccacca tgatctacaa gtgccccatg tgtagggaat ttttctctga gagagcagat
       61 ctttttatgc atcagaaaat tcacacagct gagaagcccc ataaatgtga caagtgtgat
      121 aagggtttct ttcatatatc agaacttcat attcattgga gagaccatac aggagagaag
      181 gtctataaat gtgatgattg tggtaaggat tttagcacta caacaaaact taatagacat
      241 aagaaaatcc acacagtgga gaagccctat aaatgttacg agtgtggcaa agccttcaat
      301 tggagctccc atcttcaaat tcatatgaga gttcatacag gtgagaaacc gtatgtctgt
      361 agtgagtgtg gaaggggctt tagtaatagt tcaaaccttt gcatgcatca gagagtccac
      421 accggagaga agccctttaa atgtgaagag tgtgggaagg ccttcaggca cacctccagc
      481 ctctgcatgc atcaaagagt ccacacagga gagaaaccct ataaatgtta tgagtgtggg
      541 aaggcgttca gtcagagttc gagcctctgc atccaccaga gagtccacac tggagagaaa
      601 ccctatagat gttgtggatg tgggaaggcc ttcagtcaga gttcgagcct gtgcatccac
      661 cagagagtcc acacaggaga gaaacctttc aaatgtgatg agtgcggaaa ggccttcagt
      721 cagagtacga gcctctgcat ccaccagaga gtccacacaa aggagagaaa ccatctcaaa
      781 atatcagtta tatagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
      841 tatatatctt gtggaaagga cgagcctagc aatgttggcg gtcagac
//
