LOCUS       sequence-281669-        1331 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2201
FEATURES             Location/Qualifiers
     source          1..1331
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             520..549
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             523..552
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             526..555
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 accgccacca tgatgatgat gtccctgaac agcaagcagg cgtttagcat gccgcacggc
       61 ggcagcctgc acgtggagcc caagtactcg gcactgcaca gcacctcgcc gggctcctcg
      121 gctcccatcg cgccctcggc cagctccccc agcagctcga gcaacgctgg tggtggcggc
      181 ggcggcggcg gcggcggcgg cggcggcgga ggccgaagca gcagctccag cagcagtggc
      241 agcagcggcg gcgggggctc ggaggctatg cggagagcct gtcttccaac cccaccgagc
      301 aatatattcg gcgggctgga tgagagtctg ctggcccgcg ccgaggctct ggcagccgtg
      361 gacatcgtct cccagagcaa gagccaccac caccatccac cccaccacag ccccttcaaa
      421 ccggacgcca cctaccacac tatgaatacc atcccgtgca cgtcggccgc ctcttcttca
      481 tcggtgccca tctcgcaccc ttccgcgttg gcgggcacgc accaccacca ccaccatcac
      541 caccaccacc accaccaacc gcaccaggcg ctggagggcg agctgctgga gcacctgagt
      601 cccgggctgg ccctgggcgc tatggcgggc cccgacggcg ctgtggtgtc cacgccggct
      661 cacgcgccgc acatggccac catgaacccc atgcaccaag cagcgctcag catggcccac
      721 gcgcacgggc tgccgtcgca catgggctgc atgagcgacg tggacgccga cccgcgggac
      781 ctggaggcat tcgccgagcg cttcaagcag cgacgcatca agctgggggt gacccaggca
      841 gatgtgggct ccgcgctggc caacctcaag atccccggcg tgggctcgct tagccagagc
      901 accatctgca ggttcgagtc cctcacactg tcccacaata atatgatcgc gctcaaaccc
      961 atcctgcagg catggctcga ggaggccgag aagtcccacc gcgagaagct caccaagcct
     1021 gaactcttca atggcgcgga gaagaagcgc aagcgcacgt ccatcgctgc gccagagaag
     1081 cgctcgctcg aagcctactt tgccattcag cctcggccct cctctgaaaa gatcgccgcc
     1141 atcgcggaga agctggacct gaagaaaaac gtggtgcgcg tctggttctg caaccagagg
     1201 cagaaacaga aaagaatgaa atattccgcc ggcatttagt aatgaactag tccgtaactt
     1261 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaata cagtccttga
     1321 gcaatatgcg c
//
