LOCUS       sequence-281672-        1514 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2218
FEATURES             Location/Qualifiers
     source          1..1514
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatcccgg gcagcagccg ccgcctcaac cggcccccca gggccaaggg
       61 cagccgcctt cgcagccccc gcaggggcag ggcccgccgt ccggacccgg gcaaccggca
      121 cccgcggcga cccaggcggc gccgcaggca ccccccgccg ggcatcagat cgtgcacgtc
      181 cgcggggact cggagaccga cctggaggcg ctcttcaacg ccgtcatgaa ccccaagacg
      241 gccaacgtgc cccagaccgt gcccatgagg ctccggaagc tgcccgactc cttcttcaag
      301 ccgccggagc ccaaatccca ctcccgacag gccagtactg atgcaggcac tgcaggagcc
      361 ctgactccac agcatgttcg agctcattcc tctccagctt ctctgcagtt gggagctgtt
      421 tctcctggga cactgacccc cactggagta gtctctggcc cagcagctac acccacagct
      481 cagcatcttc gacagtcttc ttttgagata cctgatgatg tacctctgcc agcaggttgg
      541 gagatggcaa agacatcttc tggtcagaga tacttcttaa atcacatcga tcagacaaca
      601 acatggcagg accccaggaa ggccatgctg tcccagatga acgtcacagc ccccaccagt
      661 ccaccagtgc agcagaatat gatgaactcg gcttcagcca tgaaccagag aatcagtcag
      721 agtgctccag tgaaacagcc accacccctg gctccccaga gcccacaggg aggcgtcatg
      781 ggtggcagca actccaacca gcagcaacag atgcgactgc agcaactgca gatggagaag
      841 gagaggctgc ggctgaaaca gcaagaactg cttcggcagg tgaggccaca ggcaatgcgg
      901 aatatcaatc ccagcacagc aaattctcca aaatgtcagg agttagccct gcgtagccag
      961 ttaccaacac tggagcagga tggtgggact caaaatccag tgtcttctcc cgggatgtct
     1021 caggaattga gaacaatgac gaccaatagc tcagatcctt tccttaacag tggcacctat
     1081 cactctcgag atgagagtac agacagtgga ctaagcatga gcagctacag tgtccctcga
     1141 accccagatg acttcctgaa cagtgtggat gagatggata caggtgatac tatcaaccaa
     1201 agcaccctgc cctcacagca gaaccgtttc ccagactacc ttgaagccat tcctgggaca
     1261 aatgtggacc ttggaacact ggaaggagat ggaatgaaca tagaaggaga ggagctgatg
     1321 ccaagtctgc aggaagcttt gagttctgac atccttaatg acatggagtc tgttttggct
     1381 gccaccaagc tagataaaga aagctttctt acatggttat agtaatgaac tagtccgtaa
     1441 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga ctaggctgga
     1501 gttcttgagg tact
//
