LOCUS       sequence-281678-        1889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2234
FEATURES             Location/Qualifiers
     source          1..1889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             979..1008
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 accgccacca tggccttgac tgacggcggc tggtgcttgc cgaagcgctt cggggccgcg
       61 ggtgcggacg ccagcgactc cagagccttt ccagcgcggg agccctccac gccgccttcc
      121 cccatctctt cctcgtcctc ctcctgctcc cggggcggag agcggggccc cggcggcgcc
      181 agcaactgcg ggacgcctca gctcgacacg gaggcggcgg ccggaccccc ggcccgctcg
      241 ctgctgctca gttcctacgc ttcgcatccc ttcggggctc cccacggacc ttcggcgcct
      301 ggggtcgcgg gccccggggg caacctgtcg agctgggagg acttgctgct gttcactgac
      361 ctcgaccaag ccgcgaccgc cagcaagctg ctgtggtcca gccgcggcgc caagctgagc
      421 cccttcgcac ccgagcagcc ggaggagatg taccagaccc tcgccgctct ctccagccag
      481 ggtccggccg cctacgacgg cgcgcccggc ggcttcgtgc actctgcggc cgcggcggca
      541 gcagccgcgg cggcggccag ctccccggtc tacgtgccca ccacccgcgt gggttccatg
      601 ctgcccggcc taccgtacca cctgcagggg tcgggcagtg ggccagccaa ccacgcgggc
      661 ggcgcgggcg cgcaccccgg ctggcctcag gcctcggccg acagccctcc atacggcagc
      721 ggaggcggcg cggctggcgg cggggccgcg gggcctggcg gcgctggctc agccgcggcg
      781 cacgtctcgg cgcgcttccc ctactctccc agcccgccca tggccaacgg cgccgcgcgg
      841 gagccgggag gctacgcggc ggcgggcagt gggggcgcgg gaggcgtgag cggcggcggc
      901 agtagcctgg cggccatggg cggccgcgag ccccagtaca gctcgctgtc ggccgcgcgg
      961 ccgctgaacg ggacgtacca ccaccaccac caccaccacc accaccatcc gagcccctac
     1021 tcgccctacg tgggggcgcc actgacgcct gcctggcccg ccggaccctt cgagaccccg
     1081 gtgctgcaca gcctgcagag ccgcgccgga gccccgctcc cggtgccccg gggtcccagt
     1141 gcagacctgc tggaggacct gtccgagagc cgcgagtgcg tgaactgcgg ctccatccag
     1201 acgccgctgt ggcggcggga cggcaccggc cactacctgt gcaacgcctg cgggctctac
     1261 agcaagatga acggcctcag ccggcccctc atcaagccgc agaagcgcgt gccttcatca
     1321 cggcggcttg gattgtcctg tgccaactgt cacaccacaa ctaccacctt atggcgcaga
     1381 aacgccgagg gtgaacccgt gtgcaatgct tgtggactct acatgaaact ccatggggtg
     1441 cccagaccac ttgctatgaa aaaagaggga attcaaacca ggaaacgaaa acctaagaac
     1501 ataaataaat caaagacttg ctctggtaat agcaataatt ccattcccat gactccaact
     1561 tccacctctt ctaactcaga tgattgcagc aaaaatactt cccccacaac acaacctaca
     1621 gcctcagggg cgggtgcccc ggtgatgact ggtgcgggag agagcaccaa tcccgagaac
     1681 agcgagctca agtattcggg tcaagatggg ctctacatag gcgtcagtct cgcctcgccg
     1741 gccgaagtca cgtcctccgt gcgaccggat tcctggtgcg ccctggccct ggcctagtaa
     1801 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1861 gacgattact gaatcgtctt tgatctctt
//
