LOCUS       sequence-281684-         701 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2244
FEATURES             Location/Qualifiers
     source          1..701
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgttcgc gctgctgcgg cccgtcggcg cgcacgtgct gtacccggac
       61 gtgcggctgc tgagcgagga cgaggagaac cgcagcgaga gcgacgcgtc ggaccagtcg
      121 ttcggctgct gcgagggccc ggaggcggcg cggcgcggcc cgggccccgg gggcgggcgg
      181 cgggcgggcg gcggcggcgg cgcgggcccc gtggtggtgg tgcgacagcg gcaggcggcc
      241 aacgcgcggg agcgggaccg cactcagagc gtgaacacgg ccttcacggc gctgcgcacg
      301 ctcatcccca ccgagccggt ggaccgcaag ctgtccaaga tcgagaccgt gcgcctggcg
      361 tccagctaca tcgcgcacct ggccaacgtg ctgctgctgg gcgactcggc cgacgacggg
      421 cagccgtgct tccgtgccgc gggcagtgcc aagggcgccg tccccgccgc cgccgacggc
      481 ggccgccagc cgcgctccat ctgcaccttc tgcctcagca accagcgcaa ggggggtggc
      541 cgtcgtgacc tggggggcag ctgcttgaag gtgagggggg tggcccccct tcgagggcca
      601 cggagatagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
      661 tcttgtggaa aggacgatca taatcctgcg caatgaagtc g
//
