LOCUS       sequence-281699-        1712 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2375
FEATURES             Location/Qualifiers
     source          1..1712
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctgagga ctggctggac tgcccggccc tgggccctgg ctggaagcgc
       61 cgcgaagtct ttcgcaagtc aggggccacc tgtggacgct cagacaccta ttaccagagc
      121 cccacaggag acaggatccg aagcaaagtt gagctgactc gatacctggg ccctgcgtgt
      181 gatctcaccc tcttcgactt caaacaaggc atcttgtgct atccagcccc caaggcccat
      241 cccgtggcgg ttgccagcaa gaagcgaaag aagccttcaa ggccagccaa gactcggaaa
      301 cgtcaggttg gaccccagag tggtgaggtc aggaaggagg ccccgaggga tgagaccaag
      361 gctgacactg acacagcccc agcttcattc cctgctcctg ggtgctgtga gaactgtgga
      421 atcagcttct caggggatgg cacccaaagg cagcggctca aaacgttgtg caaagactgt
      481 cgagcacaga gaattgcctt caaccgggaa cagagaatgt ttaagcgtgt gggctgtggg
      541 gagtgtgcag cctgccaggt aacagaagac tgtggggcct gctccacctg cctcctgcag
      601 ctgccccatg atgtggcatc ggggctgttc tgcaagtgtg aacggagacg ctgcctccgg
      661 attgtggaaa ggagccgagg gtgtggagta tgccggggct gtcagaccca agaggattgt
      721 ggccattgcc ccatctgcct tcgccctccc cgccctggtc tcaggcgcca gtggaaatgt
      781 gtccagcgac gttgcctacg gggtaaacat gcccgccgca agggaggctg tgactccaag
      841 atggctgcca ggcggcgccc cggagcccag ccactgcctc caccaccccc atcacagtcc
      901 ccagagccca cagagccgca gccctacacg aaccgccggc agaaccgcaa gtgcggggcc
      961 tgtgcagcct gcctacggcg gatggactgt ggccgctgcg acttctgctg cgacaagccc
     1021 aaattcgggg gcagcaacca gaagcgccag aagtgtcgtt ggcgccaatg cctgcagttt
     1081 gccatgaagc ggctgctgcc cagtgtctgg tcagagtctg aggatggggc aggatcgccc
     1141 ccaccttacc gtcgtcgaaa gaggcccagc tctgcccgac ggcaccatct tggccctacc
     1201 ttgaagccca ccttggctac acgcacagcc caaccagacc atacccaggc tccaacgaag
     1261 caggaagcag gtggtggctt tgtgctgccc ccgcctggca ctgaccttgt gtttttacgg
     1321 gaaggcgcaa gcagtcctgt gcaggtgccg ggccctgttg cagcttccac agaagccctg
     1381 ttgcaggcag tagacccagg cctgccttct gtgaagcaag agccacctga cccagaggag
     1441 gacaaggagg agaacaagga tgattctgcc tccaaattgg ccccagagga agaggcagga
     1501 ggggctggca cacccgtgat cacggagatt ttcagcctgg gtggaacccg cttccgagat
     1561 acagcagtct ggttgccaag gtccaaagac cttaaaaaac ctggagctag aaagcagtag
     1621 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1681 aaggacgaca cgctgccggc atcatagaag ct
//
