LOCUS       sequence-281705-        1385 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2457
FEATURES             Location/Qualifiers
     source          1..1385
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggaacccc aaagccacgg atcctgccct ggctggtgtc gcagctggac
       61 ctggggcaac tggagggcgt ggcctgggtg aacaagagcc gcacgcgctt ccgcatccct
      121 tggaagcacg gcctacggca ggatgcacag caggaggatt tcggaatctt ccaggcctgg
      181 gccgaggcca ctggtgcata tgttcccggg agggataagc cagacctgcc aacctggaag
      241 aggaatttcc gctctgccct caaccgcaaa gaagggttgc gtttagcaga ggaccggagc
      301 aaggaccctc acgacccaca taaaatctac gagtttgtga actcaggagt tggggacttt
      361 tcccagccag acacctctcc ggacaccaat ggtggaggca gtacttctga tacccaggaa
      421 gacattctgg atgagttact gggtaacatg gtgttggccc cactcccaga tccgggaccc
      481 ccaagcctgg ctgtagcccc tgagccctgc cctcagcccc tgcggagccc cagcttggac
      541 aatcccactc ccttcccaaa cctggggccc tctgagaacc cactgaagcg gctgttggtg
      601 ccgggggaag agtgggagtt cgaggtgaca gccttctacc ggggccgcca agtcttccag
      661 cagaccatct cctgcccgga gggcctgcgg ctggtggggt ccgaagtggg agacaggacg
      721 ctgcctggat ggccagtcac actgccagac cctggcatgt ccctgacaga caggggagtg
      781 atgagctacg tgaggcatgt gctgagctgc ctgggtgggg gactggctct ctggcgggcc
      841 gggcagtggc tctgggccca gcggctgggg cactgccaca catactgggc agtgagcgag
      901 gagctgctcc ccaacagcgg gcatgggcct gatggcgagg tccccaagga caaggaagga
      961 ggcgtgtttg acctggggcc cttcattgta gatctgatta ccttcacgga aggaagcgga
     1021 cgctcaccac gctatgccct ctggttctgt gtgggggagt catggcccca ggaccagccg
     1081 tggaccaaga ggctcgtgat ggtcaaggtt gtgcccacgt gcctcagggc cttggtagaa
     1141 atggcccggg tagggggtgc ctcctccctg gagaatactg tggacctgca catttccaac
     1201 agccacccac tctccctcac ctccgaccag tacaaggcct acctgcagga cttggtggag
     1261 ggcatggatt tccagggccc tggggagagc tagtaatgaa ctagtccgta acttgaaagt
     1321 atttcgattt cttggcttta tatatcttgt ggaaaggacg aacggcgact attgaacaat
     1381 ttagc
//
