LOCUS       sequence-281711-        1589 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2709
FEATURES             Location/Qualifiers
     source          1..1589
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctaaaag accgggaccc cctggaagcc gagaaatggg actgttgaca
       61 ttcagagaca tagctataga attttctctg gcggaatggc aatgcctgga tcatgctcag
      121 cagaatttat atagagatgt gatgttagag aactacagaa acctggtctc cctgggtatt
      181 gctgtctcta agccagactt gatcacctgt ctggagcaaa ataaagagcc ccagaatata
      241 aagagaaatg agatggtagc caaacaccca gttacatgtt ctcatttcac ccaagacctt
      301 cagtcagagc agggcataaa agattcactc caaaaagtga tactgagaag atatggaaaa
      361 tgtggacagg aggatttaca agtaaaaaaa tgctgtaaaa gtgtaggtga gtgtgaggtg
      421 cacaaaggag gttataatta tgttaaccaa tgtttgtcag ctacccaaaa caaaacattt
      481 cagactcata aatgcgtcaa agtctttggt aaattttcaa attccaatag acacaagaca
      541 agacatactg gaaagaaaca tttcaaatgt aaaaacgatg gcaaatcatt ttgcatgctt
      601 tcacgcctaa atcaacatca gataattcat actagggaga agtcttacaa atgtgaagaa
      661 tgtggcaaat cctttaactg ctcttcaacc cttactagac ataaaagaat tcatactgga
      721 gagaaaccct acagatgtga ggaatgtggc aaagctttta gctggtctgc atcccttact
      781 aaacataaga gaattcatac tggagagaaa ccttacacat gtgaagaacg tggcaaagtc
      841 tttagccgct caacacttac taactacaag agaattcata ctggagagaa accctacaca
      901 tgtgaagaat gtggcaaagc ctttagccgc tcttcaacac ttactaacca caagagaatt
      961 catactggag agagacccta caaatgtgaa gaatgtggca aagcctttag cttatcctca
     1021 acccttaaga aacataagat agttcatact ggggagaaac tctacacatg tgaagaatgt
     1081 gggaaagcct ttaccttctc ctcaactcta aatactcata agaggattca tactggagag
     1141 aaaccctaca cttgtgaaga atgtggcaaa gcctttagct taccctcaac cttcacttac
     1201 cacaagagaa ctcatactgg agagaaaccc tacaaatgtg aagaatgtgg caaagccttt
     1261 aactgctcct caacccttaa gaaacataag ataattcata ctggagagaa actctacaaa
     1321 tgtaaagaat gtgggaaagc ctttaccttc tcctcaactc taaatactca taagaggatt
     1381 catactggag agaaacccta caaatgtgaa gaatgtgacc aaacttttaa gtggcattca
     1441 agtcttgcta atcataagaa tatgcatact ggagagaaac cctacaaata tgaatagtaa
     1501 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1561 gacgagctct atgggtaacc cagtgttcg
//
