LOCUS       sequence-281720-        2504 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2800
FEATURES             Location/Qualifiers
     source          1..2504
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggcgct ggcggaggag cagacggagg tggcggtcaa gctagagcct
       61 gagggaccgc caacgctgct acctccgcag gcgggggacg gcgcaggcga gggtagcggc
      121 ggcactacca acaacggccc caacggcggc ggcgggaacg ttgcggcgtc gtcgtccact
      181 ggcggggatg gcgggacccc caagcccacg gtggctgtct ccgccgctgc cccggcgggg
      241 gcggccccgg tgcccgccgc tgctccggac gccggcgctc cgcatgaccg acagactcta
      301 ctggccgtgc tgcagttcct acggcagagc aaactccgcg aggccgaaga ggcgctgcgc
      361 cgtgaggccg ggctgctgga ggaggcagtg gcgggctccg gagccccggg agaggtggac
      421 agcgccggcg ctgaggtgac cagcgcgctt ctcagccggg tgaccgcctc ggcccctggc
      481 cctgcggccc ccgaccctcc gggcactggc gcttcggggg ccacggtcgt ctcaggttca
      541 gcctcaggtc ctgcggctcc gggtaaagtt ggaagtgttg ctgtggaaga ccagccagat
      601 gtcagtgccg tgttgtcagc ctacaaccaa caaggagatc ccacaatgta tgaagaatac
      661 tatagtggac tgaaacactt cattgaatgt tccctggact gccatcgggc agagttgtcc
      721 caactttttt atcctctgtt tgtgcacatg tacttggagc tagtctacaa tcaacatgag
      781 aatgaagcaa agtcattctt tgagaagttc catggagatc aggaatgtta ttaccaggat
      841 gacctacgag tattatctag tcttaccaaa aaggaacaca tgaaagggaa tgagaccatg
      901 ttggattttc gaacaagtaa atttgttctg cgtatttccc gtgactcgta ccaactcttg
      961 aagaggcatc ttcaggagaa acagaacaat cagatatgga acatagttca ggagcacctc
     1021 tacattgaca tctttgatgg gatgccgcgt agtaagcaac agatagatgc gatggtggga
     1081 agtttggcag gagaggctaa acgagaggca aacaaatcaa aggtattttt tggtttatta
     1141 aaagaaccag aaattgaggt acctttggat gacgaggatg aagagggaga aaatgaagaa
     1201 ggaaaaccta aaaagaagaa gcctaaaaaa gatagtattg gatccaaaag caaaaaacaa
     1261 gatcccaatg ctccacctca gaacagaatc cctcttcctg agttgaaaga ttcagataag
     1321 ttggataaga taatgaatat gaaagaaacc accaaacgag tgcgccttgg gccggactgc
     1381 ttaccctcca tttgtttcta tacatttctc aatgcttacc agggtctcac tgcagtggat
     1441 gtcactgatg attctagtct gattgctgga ggttttgcag attcaactgt cagagtgtgg
     1501 tcggtaacac ccaaaaagct tcgtagtgtc aaacaagcat cagatcttag tcttatagac
     1561 aaagaatcag atgatgtctt agaaagaatc atggatgaga aaacagcaag tgagttgaag
     1621 attttgtatg gtcacagtgg gcctgtctac ggagccagct tcagtccgga taggaactat
     1681 ctgctttcct cttcagagga cggaactgtt agattgtgga gccttcaaac atttacttgt
     1741 ttggtgggat ataaaggaca caactatcca gtatgggaca cacaattttc tccatatgga
     1801 tattattttg tgtcaggggg ccatgaccga gtagctcggc tctgggctac agaccactat
     1861 cagcctttaa gaatatttgc cggccatctt gctgatgtga attgtaccag attccatcca
     1921 aattctaatt atgttgctac gggctctgca gacagaactg tgcggctctg ggacgtcctg
     1981 aatggtaact gtgtaaggat cttcactgga cacaagggac caattcattc cttgacattt
     2041 tctcccaatg ggagattcct ggctacagga gcaacagatg gcagagtgct tctttgggat
     2101 attggacatg gtttgatggt tggagaatta aaaggccaca ctgatacagt ctgttcactt
     2161 aggtttagta gagatggtga aattttggca tcaggttcaa tggataatac agttcgatta
     2221 tgggatgcta tcaaagcctt tgaagattta gagaccgatg actttactac agccactggg
     2281 catataaatt tacctgagaa ttcacaggag ttattgttgg gaacatatat gaccaaatca
     2341 acaccagttg tacaccttca ttttactcga agaaacctgg ttctagctgc aggagcttat
     2401 agtccacaat agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     2461 atatcttgtg gaaaggacga agatgatcac cctggattag cgtt
//
