LOCUS       sequence-281726-        1361 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2821
FEATURES             Location/Qualifiers
     source          1..1361
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggtgaaaa aaaaccagag cctttggact tcgtgaaaga ttttcaggaa
       61 tacctgactc agcagaccca tcacgtgaac atgatttctg gatcagttag tggggacaaa
      121 gaagcagagg ctcttcaggg agctggaaca gatggtgatc aaaatggact tgatcaccca
      181 tctgttgaag tttccttgga tgaaaactca ggaatgttag tagacgggtt tgaaaggacc
      241 tttgatggga agcttaagtg tcggtactgc aactatgcca gcaaaggaac agcccggctt
      301 attgaacaca tcagaatcca cacaggtgaa aaacctcatc gatgtcatct ttgtccattt
      361 gcatctgctt atgagcgtca tctggaagcc catatgcgtt ctcatactgg agaaaaacca
      421 tacaaatgtg aattatgttc cttccgctgc agtgatcgaa gtaacttgtc ccatcatcga
      481 aggcgcaagc ataaaatggt accaattaaa ggtactaggt cttccttaag cagcaagaaa
      541 atgtgggggg ttttacagaa gaaaacaagc aatctgggct atagcagaag agcactaatc
      601 aacttaagtc caccttccat ggtggttcag aaaccagact accttaacga ttttacccac
      661 gaaatcccaa atatccagac tgactcctat gaaagtatgg caaaaaccac accaactggt
      721 ggccttccaa gggaccccca agaactcatg gttgataacc ctttgaatca gctctcgact
      781 ctagcagggc agttgtccag tctgccaccc gaaaaccaaa accctgcatc ccctgatgta
      841 gttccctgcc ctgatgaaaa gcctttcatg attcagcagc cctctaccca agcagtagtt
      901 tctgccgtat cagcaagtat tcctcagagc tcctctccca caagcccaga acctcggcca
      961 tcccatagtc aaaggaacta tagtccagtg gcaggtccaa gcagtgagcc aagtgcccac
     1021 acgagcactc ccagcatagg aaacagccag ccaagcaccc cagccccagc cctgccggtc
     1081 caggaccctc agcttctgca ccactgccag cactgtgata tgtactttgc agacaacatc
     1141 ctttacacta ttcatatggg atgtcatggg tatgaaaatc cttttcagtg taatatatgt
     1201 ggatgcaaat gtaaaaacaa gtatgatttt gcctgtcatt ttgcaagagg gcaacataac
     1261 caacattagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1321 tcttgtggaa aggacgaaga agttcctaat ttctgtgagt a
//
