LOCUS       sequence-281729-        1793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0009
FEATURES             Location/Qualifiers
     source          1..1793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggaccatt gacatttatg gatgtggcca tagaattctc tctggaggag
       61 tggcagtgcc tggacacagc acagcagaat ttatatagga atgtcatgtt agagaattat
      121 agaaacctgg tattcctggg tattgctgtg tctaatttag acctgatcac ctgtctggag
      181 caaggaaaag agccctggaa tatgaagaga cacgagatgg cagccaaacc cccagctatg
      241 tgttctcatt ttgccaaaga ccttaggcca gagcaatata taaaaaattc tttccaacaa
      301 gtgatactga gaagatatgg aaaatgtgga tatcagaaag gctgtaaaag tgtggatgag
      361 cataagttgc acaaaggagg tcacaaggga cttaaccggt gtgtgacaac tacccagagc
      421 aaaatagttc agtgtgacaa atacgtgaaa gtctttcata aatattcaaa tgcaaagaga
      481 cataagataa gacatactgg aaaaaatcct ttcaaatgta aagaatgtgg caaatcattt
      541 tgcatgcttt cacaactaac tcaacatgag ataattcata ctggagaaaa accctacaaa
      601 tgtgaagaat gtggcaaagc ttttaaaaag tcctcaaacc ttacgaatca taagataatt
      661 catactggag agaaacccta caaatgtgaa gaatgtggaa aagcttttaa ccagtcctca
      721 actcttacta gacataagat aattcatact ggagagaaac tctacaaatg tgaagaatgt
      781 ggcaaagctt ttaaccggtc ctcaaacctt actaaacata agatagttca tactggagag
      841 aaaccctaca aatgtgaaga atgtggcaaa gcttttaaac agtcctcaaa ccttactaat
      901 cacaagaaaa ttcatactgg agagaaaccc tacaaatgtg gagaatgtgg caaagccttt
      961 accctatctt cacaccttac tacacataag aggattcata ctggtgagaa accctacaaa
     1021 tgtgaagaat gtggcaaagc ttttagtgta ttttcaaccc ttactaaaca taagataatt
     1081 catactgaag agaaacccta caaatgtgaa gaatgtggca aagcttttaa ccggtcctca
     1141 caccttacta atcataaggt aattcatact ggagagaaac cctacaaatg tgaagaatgt
     1201 ggtaaagcct ttaccaagtc ctcaactctt acttatcata aggtaattca tactggaaag
     1261 aaaccctaca aatgtgaaga atgtggtaaa gcctttagta tattctcaat ccttactaaa
     1321 cataaagtaa ttcatactga agacaaaccc tacaaatgtg aagaatgtgg caaaactttt
     1381 aactactcct caaattttac taatcataaa aaaattcata ctggagagaa accctataag
     1441 tgtgaagaat gtggcaaaag ctttattctg tcctctcatc ttactacaca taagataatt
     1501 catactggag agaaacccta caaatgtaaa gaatgtggca aagcttttaa ccagtcctca
     1561 acccttatga aacataagat aattcatact ggagagaaac cctacaaatg tgaagaatgt
     1621 ggcaaagcct ttaaccagtc cccaaacctt actaaacata agagaattca taccaaagag
     1681 aaaccctaca aatgtaaata gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1741 tggctttata tatcttgtgg aaaggacgat cgcctcgcgc acttggcccg atc
//
