LOCUS       sequence-281735-        2531 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0030
FEATURES             Location/Qualifiers
     source          1..2531
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggaccatt gacatttatg gatgtggcca tagaattctg tctggaggag
       61 tggcaatgcc tggacattgc acagcagaat ttatatagga atgtgatgtt agagaactac
      121 agaaacctgg tcttcctggg tattgctgtc tctaagccag acctgatcac ctgtctggag
      181 caagaaaaag agccttggga gcctatgagg agacatgaaa tggtagccaa acccccagtt
      241 atgtgttctc attttaccca agacttttgg ccagagcagc atataaaaga tcctttccaa
      301 aaagcgacac tgagaagata taaaaactgt gaacataaaa atgtacattt aaaaaaagac
      361 cataaaagtg tggatgagtg taaggtgcac agaggaggtt ataatggatt taaccaatgt
      421 ttgccagcta cccagagcaa aatatttcta tttgataaat gtgtgaaagc ctttcataaa
      481 ttttcaaatt caaacagaca taagataagc catactgaaa aaaaactttt caaatgcaaa
      541 gaatgtggca aatcattttg catgcttcca catctagctc aacataaaat aattcatacc
      601 agagtgaatt tctgcaaatg tgaaaaatgt ggaaaagctt ttaactgccc ttcaatcatc
      661 actaaacata agagaattaa tactggagag aaaccctaca catgtgaaga atgtggcaaa
      721 gtctttaatt ggtcctcacg ccttactaca cataaaaaaa attatactag atacaaactc
      781 tacaaatgtg aagaatgtgg caaagctttt aacaagtcct caatccttac tacccataag
      841 ataattcgca ctggagagaa attctacaaa tgtaaagaat gtgccaaagc ttttaaccaa
      901 tcctcaaacc ttactgaaca taagaaaatt catcctggag agaaacctta caaatgtgaa
      961 gaatgtggca aagcctttaa ctggccctca actcttacta aacataagag aattcatact
     1021 ggagagaaac cctacacatg tgaagaatgt ggcaaagcct ttaaccagtt ctcaaacctt
     1081 actacacata agagaatcca tactgcagag aaattctata aatgtacaga atgtggtgaa
     1141 gcttttagcc ggtcctcaaa ccttactaaa cataagaaaa ttcatactga aaagaaaccc
     1201 tacaaatgtg aagaatgtgg caaagctttt aagtggtcct caaagcttac tgaacataag
     1261 ttaactcata ctggagagaa accctacaaa tgtgaagaat gtggcaaagc ctttaactgg
     1321 ccctcaaccc ttactaaaca taacagaatt catactggag agaaacccta caaatgtgaa
     1381 gtatgtggca aagcctttaa ccagttctca aaccttacta cacataagag aattcatact
     1441 gcagaaaaac cgtacaaatg tgaagaatgt ggcaaagctt ttagccggtc ctcaaacctt
     1501 actaaacata agaaaattca cattgaaaag aaaccctaca aatgtgaaga atgtggcaaa
     1561 gcttttaagt ggtcctcaaa gcttactgaa cataagataa ctcatactgg agagaaaccc
     1621 tacaaatgtg aagaatgtgg caaagctttt aaccatttct caatccttac caaacataag
     1681 aggattcata ctggagagaa accctacaag tgtgaagaat gtggcaaagc ttttacccaa
     1741 tcctcaaacc ttactacaca taagaaaatt catactggag agaaattcta caaatgtgaa
     1801 gaatgtggca aagcttttac ccaatcttca aaccttacta cacataaaaa aattcatact
     1861 ggaggaaaac cctacaaatg tgaagaatgt ggcaaagctt ttaaccagtt ctcaactctt
     1921 actaaacata agataattca cactgaggag aaaccctaca aatgtgaaga atgtggcaaa
     1981 gcctttaagt ggtcctcaac ccttactaaa cataagataa ttcatactgg agagaaaccc
     2041 tacaaatgtg aagaatgtgg caaagctttt aaactgtcct caaccctttc tacacataag
     2101 attattcata ctggagagaa accctacaaa tgtgaaaaat gtggcaaagc ttttaaccga
     2161 tcctcaaacc ttattgaaca taagaaaatt catactggag agcaacccta caaatgtgaa
     2221 gaatgtggca aagcatttaa ctattcctca caccttaata cacataagag aattcatact
     2281 aaagagcaac cctacaaatg taaagaatgt ggcaaagctt tcaaccaata ttcaaacctt
     2341 actacacata acaaaattca tactggagag aaactctaca aacctgaaga tgtgacagtg
     2401 attttgacaa cacctcaaac tttttcaaac ataaaatagt aatgaactag tccgtaactt
     2461 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgattc cggatggtgc
     2521 caacctatac a
//
