LOCUS       sequence-281738-        2513 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0031
FEATURES             Location/Qualifiers
     source          1..2513
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatgtggc catagaattc tgtctggagg agtggcaatg cctggacatt
       61 gcacagcaga atttatatag gaatgtgatg ttagagaact acagaaacct ggtcttcctg
      121 ggtattgctg tctctaagcc agacctgatc acctgtctgg agcaagaaaa agagccttgg
      181 gagcctatga ggagacatga aatggtagcc aaacccccag ttatgtgttc tcattttacc
      241 caagactttt ggccagagca gcatataaaa gatcctttcc aaaaagcgac actgagaaga
      301 tataaaaact gtgaacataa aaatgtacat ttaaaaaaag accataaaag tgtggatgag
      361 tgtaaggtgc acagaggagg ttataatgga tttaaccaat gtttgccagc tacccagagc
      421 aaaatatttc tatttgataa atgtgtgaaa gcctttcata aattttcaaa ttcaaacaga
      481 cataagataa gccatactga aaaaaaactt ttcaaatgca aagaatgtgg caaatcattt
      541 tgcatgcttc cacatctagc tcaacataaa ataattcata ccagagtgaa tttctgcaaa
      601 tgtgaaaaat gtggaaaagc ttttaactgc ccttcaatca tcactaaaca taagagaatt
      661 aatactggag agaaacccta cacatgtgaa gaatgtggca aagtctttaa ttggtcctca
      721 cgccttacta cacataaaaa aaattatact agatacaaac tctacaaatg tgaagaatgt
      781 ggcaaagctt ttaacaagtc ctcaatcctt actacccata agataattcg cactggagag
      841 aaattctaca aatgtaaaga atgtgccaaa gcttttaacc aatcctcaaa ccttactgaa
      901 cataagaaaa ttcatcctgg agagaaacct tacaaatgtg aagaatgtgg caaagccttt
      961 aactggccct caactcttac taaacataag agaattcata ctggagagaa accctacaca
     1021 tgtgaagaat gtggcaaagc ctttaaccag ttctcaaacc ttactacaca taagagaatc
     1081 catactgcag agaaattcta taaatgtaca gaatgtggtg aagcttttag ccggtcctca
     1141 aaccttacta aacataagaa aattcatact gaaaagaaac cctacaaatg tgaagaatgt
     1201 ggcaaagctt ttaagtggtc ctcaaagctt actgaacata agttaactca tactggagag
     1261 aaaccctaca aatgtgaaga atgtggcaaa gcctttaact ggccctcaac ccttactaaa
     1321 cataacagaa ttcatactgg agagaaaccc tacaaatgtg aagtatgtgg caaagccttt
     1381 aaccagttct caaaccttac tacacataag agaattcata ctgcagaaaa accgtacaaa
     1441 tgtgaagaat gtggcaaagc ttttagccgg tcctcaaacc ttactaaaca taagaaaatt
     1501 cacattgaaa agaaacccta caaatgtgaa gaatgtggca aagcttttaa gtggtcctca
     1561 aagcttactg aacataagat aactcatact ggagagaaac cctacaaatg tgaagaatgt
     1621 ggcaaagctt ttaaccattt ctcaatcctt accaaacata agaggattca tactggagag
     1681 aaaccctaca agtgtgaaga atgtggcaaa gcttttaccc aatcctcaaa ccttactaca
     1741 cataagaaaa ttcatactgg agagaaattc tacaaatgtg aagaatgtgg caaagctttt
     1801 acccaatctt caaaccttac tacacataaa aaaattcata ctggaggaaa accctacaaa
     1861 tgtgaagaat gtggcaaagc ttttaaccag ttctcaactc ttactaaaca taagataatt
     1921 cacactgagg agaaacccta caaatgtgaa gaatgtggca aagcctttaa gtggtcctca
     1981 acccttacta aacataagat aattcatact ggagagaaac cctacaaatg tgaagaatgt
     2041 ggcaaagctt ttaaactgtc ctcaaccctt tctacacata agattattca tactggagag
     2101 aaaccctaca aatgtgaaaa atgtggcaaa gcttttaacc gatcctcaaa ccttattgaa
     2161 cataagaaaa ttcatactgg agagcaaccc tacaaatgtg aagaatgtgg caaagcattt
     2221 aactattcct cacaccttaa tacacataag agaattcata ctaaagagca accctacaaa
     2281 tgtaaagaat gtggcaaagc tttcaaccaa tattcaaacc ttactacaca taacaaaatt
     2341 catactggag agaaactcta caaacctgaa gatgtgacag tgattttgac aacacctcaa
     2401 actttttcaa acataaaata gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     2461 tggctttata tatcttgtgg aaaggacgag cctcatccag ttggcgaagt gtt
//
