LOCUS       sequence-281750-        2102 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0196
FEATURES             Location/Qualifiers
     source          1..2102
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacttggt ggctttcgag gatgtggctg tgaacttcac ccaggaggag
       61 tggtctttgc tggatccttc ccagaagaat ctctacagag aagtgatgca ggaaaccttg
      121 aggaatctgg cctccatagg agaaaaatgg aaagaccaga acattgaaga tcagtacaaa
      181 aatcccagga ataatctaag aagtcttctg ggagagagag ttgatgaaaa tacagaagaa
      241 aatcattgtg gagaaacttc tagccagatt ccagatgaca cactgaacaa aaaaacttct
      301 cctggagtaa aatcatgtga aagcagtgtg tgtggagaag tcttcgtggg tcattcttcc
      361 cttaataggc acattagagc tgacactgca cacaagccgt ctgagtatca ggaatatgga
      421 caggagccat ataagtgtca acaacgtaag aaagccttca gatgtcaccc ctcctttcaa
      481 atgcaagaaa aggctcacac tggagaaaaa ctctatgatt gtaaagaatg tggaaaaacc
      541 ttcatatccc attcaagcat tcaaagacac atgataatgc acaatggaga tggaacttat
      601 aaatgtaagt tttgtgggaa agcctgccct tgtctcagca tatatcttat acatgaacga
      661 gttcacactg gagagaaacc atataaatgt aaacaatgtg gtaaagcctt tagttattca
      721 acttcccttc aaatacatga aagaactcac actggagaga agccttatga atgtaaggaa
      781 tgtgggaaag cattcggtag tcccaattcc ctttatgaac atagaagaac tcacactgga
      841 gagaagccat atgaatgcaa acaatgtgga aaagccttca gatggttcca ttcctttcaa
      901 atacatgaaa gaactcacag tgaggagaag gcttatgaat gtaccaaatg tgggaaagca
      961 ttcaagtgtc ccagttatct ttgtagacat gaagtgaccc actctgggaa aaagccctgt
     1021 gaatgtaaac aatgtgggaa agcattatct tatcttaact ttcaaagaca catgaaaatg
     1081 cacactagaa tgagacctta taaatgtaag actgtggaaa agcctttgat tctcccagtt
     1141 cgttttgaag acatgaaaga actcacactg gagagaaacc ttatgaatgc aagcactgtg
     1201 gtaaagcctt caatcgttcc agttcctttc actatcatga aaggactcac actggagaga
     1261 aaccctatga atgtaagcag tgtagtaaag ccttcatttc ttccacttcc tttcgatatc
     1321 atgaaaggac tcacactgga gagaaaccgt atgagtgtaa gcaatgtggg aaagccttca
     1381 gatctgcctc acaccttcaa atgcatggaa ggactcacac tcaagagaaa ccctatgaat
     1441 gtaagcagtg tggtaaagcc ttcatttttt ccacttcctt tcgatatcat gaaaggactc
     1501 acactggaga gaaaccctat gagtgtaagc aatgtgggaa agccttcaca tctgcctcac
     1561 accttcaaat gcatgaaagg actcacactg gaaagcaact gtatgaatct aaacaatgtg
     1621 aaaaaacctt tggatctgtc agaaaccttc aaattcatga aaagacacac cctggagaga
     1681 aaccctataa ggaatatgga aaagcattca acaatttctc ttcctttcaa atacatgcaa
     1741 caatgcacag aggacagaat gcctatgaat gtaaagagtg tgacaaagca ttcatatctg
     1801 ccaagatcct tcgagtacat gcaagaacac accctggaga gaaaccctat gaatgtaagg
     1861 aatgcggaaa agcgttcaat tatttttctt ctttgcgtat acacaaaagg atgcacactg
     1921 gagagaaacc atattaatgt aaggattgtg ggaaagcatt cagtttgcct ggttcctttc
     1981 gtagacataa aagggctcac actggagtag taatgaacta gtccgtaact tgaaagtatt
     2041 tcgatttctt ggctttatat atcttgtgga aaggacgagt ttaatgtaat gtcttacacg
     2101 cg
//
