LOCUS       sequence-281759-        2048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0231
FEATURES             Location/Qualifiers
     source          1..2048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaactcgt aacattcagg gatgtggcca tagaattctc ccctgaagag
       61 tggaaatgtc tggaccctgc ccagcagaat ttgtatagag atgtgatgtt ggagaactac
      121 aggaacctgg tctccctggg ttttgtgatc tctaacccag acctggtcac ctgtctggag
      181 caaataaaag agccctgcaa tttgaagata catgagacag cagccaaacc cccagctata
      241 tgttctcctt tcagccaaga cctttcacca gtgcagggga tagaagattc attccacaaa
      301 cttatactga aaagatacga gaaatgtgga catgagaatt tacaattaag aaaaggctgt
      361 aaacgtgtga atgagtgtaa ggtgcagaaa ggagttaata atggagttta ccagtgcttg
      421 tcaactaccc agagcaaaat atttcaatgt aatacatgtg ttaaagtttt tagtaaattt
      481 tcaaattcaa acaaacataa gataagacat actggagaga aaccctttaa atgtacagaa
      541 tgtggcagat cgttttacat gtcacaccta actcaacata caggaattca tgctggagag
      601 aaaccctaca aatgtgaaaa atgtggcaaa gcctttaata ggtccacatc acttagtaaa
      661 cataagagaa ttcatactgg agagaaaccc tacacatgtg aagaatgtgg caaagccttt
      721 agacggtcca cagttctgaa cgaacataag aaaattcata ctggagagaa accctacaaa
      781 tgtgaagaat gtggcaaagc ctttacaagg tccacaacac tgaatgaaca caagaaaatt
      841 catactggag agaaacccta caaatgtaaa gaatgtggca aagcctttag atggtccaca
      901 agcctgaatg aacataagaa tattcatact ggagagaaac cctacaaatg taaagaatgt
      961 ggcaaagcct ttagacagtc caggagcctg aatgaacata aaaatattca tactggcgaa
     1021 aaaccctaca catgtgaaaa atgtggcaaa gcttttaacc aatcctcaag tcttattata
     1081 cacaggagca ttcattctga acaaaaactt tacaaatgtg aagaatgtgg caaagccttt
     1141 acttggtcct catcccttaa taaacataag agaattcata ctggagagaa accctacaca
     1201 tgtgaagaat gtggcaaagc tttttatagg tcctcacacc ttgctaaaca taagagaatt
     1261 catactggag agaaacccta cacgtgcgaa gaatgtggca aagcttttaa ccaatcctca
     1321 actcttatat tacacaagag aatccattct gggcaaaaac cttacaaatg tgaagaatgt
     1381 ggcaaagcct ttacacggtc cacaacactg aacgaacata agaaaattca tactggcgag
     1441 aaaccctaca aatgtgaaga atgtggcaaa gctttcatat ggtccgcaag cctgaatgaa
     1501 cataagaata ttcatactgg agagaaaccc tacaaatgta aagaatgtgg caaagctttt
     1561 aaccaatcct caggccttat tatacacagg agcattcatt ctgaacaaaa actttacaaa
     1621 tgtgaagaat gtggcaaagc ctttactcgg tccacagccc tgaatgaaca taagaaaatt
     1681 cattctggag agaaacccta caaatgcaaa gaatgtggca aagcctataa cttatcctca
     1741 acccttacta aacataagag aattcatact ggagagaaac ccttcacatg tgaagaatgt
     1801 ggcaaagcct tcaattggtc ctcatccctt actaaacata agataattca tactggagag
     1861 aaatcctaca aatgtgaaga atgtggcaaa gcttttaatc ggccctcaac ccttactgta
     1921 cacaagcgaa ttcatactgg caaggaacat agttagtaat gaactagtcc gtaacttgaa
     1981 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaatctc ggttaagggt
     2041 atatagga
//
