LOCUS       sequence-281780-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0437
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggtgacaca gctcagatat gaatcttttt gcctccacca atttcaagaa
       61 caagatggtg aaagtatacc tgagaaccag gagttggcat caaagcaaga aatcttaaaa
      121 gaaatggaac atttggggga tagcaaactc caaagagatg tatctttgga ttctaagtac
      181 agagaaactt gtaaacgaga cagcaaggca gaaaagcagc aggcacattc cactggagag
      241 agacgccaca ggtgcaatga atgtgggaaa agcttcacta agagttcagt actcattgag
      301 caccagagaa tccacactgg ggagaagcca tatgaatgtg aagaatgtgg gaaggccttc
      361 agccggaggt caagcctgaa tgaacatcgg cggagccaca ctggagagaa accctatcaa
      421 tgtaaggagt gtgggaaagc cttcagtgcc agcaatggcc tcactcgaca cagaagaatc
      481 cacacagggg aaaaaccata tgaatgcaaa gtgtgtggga aggctttcct cctcagctca
      541 tgccttgttc agcatcagag gatacacact ggagagaagc gctatcagtg tcgtgagtgt
      601 ggcaaagcct tcattcagaa tgcagggctt ttccagcatc tccgagtcca cactggtgag
      661 aaaccctatc agtgcagtca gtgcagtaaa ctctttagta agcggacact tcttaagaaa
      721 catcagaaaa tccacactgg agagagacca tagtaatgaa ctagtccgta acttgaaagt
      781 atttcgattt cttggcttta tatatcttgt ggaaaggacg agttgcggaa ctcctgcccg
      841 cgggc
//
