LOCUS       sequence-281786-        1985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0446
FEATURES             Location/Qualifiers
     source          1..1985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgatggc tttgacggac ccggcgcagg tgtctgtgac ctttgatgat
       61 gtggctgtga ctttcaccca ggaggagtgg ggccagctgg acctagctca gcggaccctg
      121 taccaggagg tgatgctgga aaactgtggg ctcctggtat ctctggggtg tcctgttccc
      181 agacctgagc tgatctacca cctagagcat gggcaggagc catggaccag gaaggaagac
      241 ctctcccaag gcacctgtcc aggtgacaaa ggaaaaccca agagcacaga acctaccacc
      301 tgtgagctag ccttgtctga aggaatctct ttttggggac aactaacaca aggagcttca
      361 ggggactccc agttggggca acccaaggat caggatgggt tttcagaaat gcagggagaa
      421 cgcttgagac cagggttaga ttcccaaaag gagaagcttc ctggaaaaat gagccccaaa
      481 catgatggtt tagggacagc tgatagtgtg tgttcaagga ttatacagga tcgagtctcc
      541 ttaggagatg atgtccatga ctgtgactca catggatcag gtaaaaatcc agttattcag
      601 gaagaggaaa atatctttaa atgcaatgaa tgtgaaaaag tgtttaacaa gaaacgcctg
      661 cttgctcggc atgagaggat tcactctgga gtgaagccct atgaatgcac agagtgtgga
      721 aaaaccttta gcaagagtac atacctcctg cagcaccaca tggtccacac tggggagaag
      781 ccctataagt gcatggagtg tgggaaggct tttaatcgga agtcacacct tacccagcac
      841 cagcggattc acagtggaga gaagccttat aagtgcagtg aatgtggaaa ggccttcacc
      901 caccgctcca cttttgtctt gcataacagg agccacactg gagaaaaacc ctttgtgtgc
      961 aaagagtgtg gcaaagcctt tcgagatagg ccaggtttca ttcgacacta catcatccac
     1021 agtggtgaga atccctacga gtgcttcgaa tgtggcaagg tcttcaaaca cagatcatac
     1081 ctcatgtggc accagcagac tcataccggg gagaagccct atgagtgcag tgaatgtggg
     1141 aaggccttct gtgagagcgc agcgctgatt caccactatg tcatccacac tggagagaag
     1201 ccctttgagt gcctcgagtg tgggaaggct ttcaaccacc gatcctacct caaaaggcac
     1261 cagcggattc acactgggga gaagccatat gtgtgtagtg aatgcggaaa ggccttcacc
     1321 cactgctcta ctttcatctt gcataaaagg gcccacactg gagaaaaacc tttcgagtgc
     1381 aaagagtgtg ggaaagcctt tagcaatagg gcagacctca ttcgccactt cagcatccac
     1441 actggagaga agccctatga gtgcatggag tgtggaaagg ccttcaaccg caggtcaggc
     1501 ctcacaaggc accagcggat tcatagtgga gagaagccct atgaatgcat cgagtgtggg
     1561 aaaacatttt gctggagcac aaacctcatt cgacactcta tcatccacac tggagagaag
     1621 ccgtatgagt gcagtgaatg tggaaaggcc ttcagtcgca gctcgtccct cactcagcat
     1681 caaaggatgc atactgggag aaatcctatc agtgtaacag atgtgggaag accttttaca
     1741 agtgggcaga cctcagtcaa catccaagaa cttttattgg ggaaaaactt tttgaatgtc
     1801 accactgagg aaaatctttt gcaagaggaa gcatcttaca tggcatctga tcgtacatac
     1861 caaagagaaa ccccacaagt gtcttcactg tagtaatgaa ctagtccgta acttgaaagt
     1921 atttcgattt cttggcttta tatatcttgt ggaaaggacg agtaaatggg acagctggct
     1981 taccc
//
