LOCUS       sequence-281795-        1694 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0485
FEATURES             Location/Qualifiers
     source          1..1694
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggtcaggcc ccaggatact gtggcgtatg aggacctgtc tgaggactat
       61 actcagaaga aatggaaagg tctcgcactc agtcagagag ccctgcactg gaacatgatg
      121 ctggaaaatg accgtagcat ggcttctttg ggtaggaaca tgatggagag ttcagagctg
      181 actccgaagc aggaaatttt taaaggatca gagtcatcta atagcacatc agggggactc
      241 tttggggtgg ttcctggggg aacagagact ggagatgttt gtgaagatac cttcaaagag
      301 ttagaaggac aaccctcaaa tgaagaaggg agcagactag aaagtgattt cttggaaata
      361 atagatgagg ataagaaaaa atccacaaaa gacagatatg aggaatataa ggaagttgag
      421 gaacatccac ctctgtcttc cagtcctgtt gaacatgaag gagttttaaa gggacagaaa
      481 tcctatcgat gtgatgaatg tggcaaagct ttttattgga gttcgcacct cattggtcat
      541 cggagaatcc acactggaga gaaaccctat gagtgtaatg agtgtgggaa gaccttcagg
      601 caaacctccc agctcattgt tcatctcaga acccacacag gggaaaagcc ctatgaatgc
      661 agtgagtgtg gaaaggccta taggcacagc tcccatctca ttcaacacca gagactccat
      721 aatggggaga aaccctataa atgtaatgaa tgtgcaaaag cttttaatca gagctccaaa
      781 ctcttcgacc accagagaac ccatactggg gagaaacctt atgaatgtaa ggagtgtggg
      841 gcggccttta gtcggagtaa aaatcttgtt cgacatcagt ttctgcacac tggtaagaaa
      901 ccttataagt gtaatgaatg tgggagagca ttctgttcca atagaaatct cattgaccat
      961 cagagaaccc acactgggga gaagccttat aaatgtaatg aatgtggcaa agccttcagt
     1021 cggagtaaat gtcttattcg acatcagagc ctccacactg gggaaaagcc atacaaatgt
     1081 agtgaatgtg ggaaagcctt caatcagatc tctcaacttg ttgaacatga gcgaattcat
     1141 actggagaaa aaccatttaa gtgtagtgag tgtggtaagg cattcggtct gagtaaatgt
     1201 cttattcggc accagaggct tcacacaagt gaaaagccct ataaatgcaa tgagtgtgga
     1261 aaatccttca atcaaaactc atacctcatt atacaccaga gaattcacac tggtgagaaa
     1321 ccctatgaat gtaatgagtg tgggaaggtc ttcagttata attctagtct tatggtacat
     1381 cagagaaccc atactgggga aaaaccctat aaatgcaata gttgtgggaa agcctttagt
     1441 gacagctcac agcttactgt gcaccagaga gtccacactg gagaaaaacc ttatgaatgt
     1501 attgagtgtg ggaaagcctt tagtcagcgt tccactttta atcaccacca gcgaactcat
     1561 gctggagaga agccctcagg tctggctcgg tcatcttctt agtaatgaac tagtccgtaa
     1621 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga gtcttaatga
     1681 ggtgtgtata ctgt
//
