LOCUS       sequence-281816-        1358 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0596
FEATURES             Location/Qualifiers
     source          1..1358
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctactca ttctcctgag aaaacccaca agtgtaatta ttgtgagaaa
       61 atgtttcacc ggaaagatca tctgaagaat cacctccata cacacgaccc taacaaagag
      121 acgtttaagt gcgaagaatg tggcaagaac tacaatacca agcttggatt taaacgtcac
      181 ttggccttgc atgccgcaac aagtggtgac ctcacctgta aggtatgttt gcaaactttt
      241 gaaagcacgg gagtgcttct ggagcacctt aaatctcatg caggcaagtc gtctggtggg
      301 gttaaagaaa aaaagcacca gtgcgaacat tgtgatcgcc ggttctacac ccgaaaggat
      361 gtccggagac acatggtggt gcacactgga agaaaggact tcctctgtca gtattgtgca
      421 cagagatttg ggcgaaagga tcacctgact cgacatatga agaagagtca caatcaagag
      481 cttctgaagg tcaaaacaga accagtggat ttccttgacc catttacctg caatgtgtct
      541 gtgcctataa aagacgagct ccttccggtg atgtccttac cttccagtga actgttatca
      601 aagccattca caaacacttt gcagttaaac ctctacaaca ctccatttca gtccatgcag
      661 agctcgggat ctgcccacca aatgatcaca actttacctt tgggaatgac atgcccaata
      721 gatatggaca ctgttcatcc ctctcaccac ctttctttca aatatccgtt cagttctacc
      781 tcatatgcaa tttctattcc tgaaaaagaa cagccattaa agggggaaat tgagagttac
      841 ctgatggagt tacaaggtgg cgtgccctct tcatcccaag attctcaagc atcgtcatca
      901 tctaagctag ggttggatcc tcagattggg tccctagatg atggtgcagg agacctctcc
      961 ctatccaaaa gctctatctc catcagtgac cccctaaaca caccagcatt ggatttttct
     1021 cagttgttta atttcatacc tttaaatggt cctccctata atcctctatc agtggggagc
     1081 cttggaatga gctattccca ggaagaagca cattcttctg tttcccagct ccccccacaa
     1141 acacaggatc ttcaggatcc tgcaaacact atagggcttg ggtctctgca ctcactgtca
     1201 gcagctttca ccagcagttt aagcacaagt accaccctcc cacgtttcca tcaagctttt
     1261 cagtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1321 tgtggaaagg acgaccgcta aaccctggat gtccgact
//
