LOCUS       sequence-281819-        1736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0599
FEATURES             Location/Qualifiers
     source          1..1736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagttat ggacctatcc tccccatggg ctttaactaa acaggactct
       61 gcctgtttcc atctgagaaa tgctgaagag gaaaggatga ttgctgtatt tctgacaacc
      121 tggttacagg aaccaatgac tttcaaagat gtagctgtgg agttcactca ggaggagtgg
      181 atgatgctgg attctgctca aagaagtctg tacagagatg tgatgttgga aaactataga
      241 aatctcacct ccgtggaata tcagttgtac aggcttactg tgatctcccc attggatcaa
      301 gaagagataa ggaatatgaa aaagagaatt ccccaagcca tctgtccaga ccagaagatt
      361 caacctaaaa ccaaagaatc aactgtgcag aaaattttgt gggaggaacc atccaatgca
      421 gtgaaaatga taaaactcac aatgcataat tggtcctcca cattaagaga agactgggaa
      481 tgccataaga tcagaaaaca gcataagatt ccagggggac attggaggca aatgatatat
      541 gccccaaaga aaacagtacc tcaggagcta ttccgtgact atcatgaatt agaggaaaac
      601 tctaaacttg gatcaaaact tattttttca cagagcattt tcaccagtaa acattgccaa
      661 aaatgttact cagagattgg atgtttgaaa cacaattcaa tcataaacaa ttatgtgaaa
      721 aactctataa gtgagaagct ctatgaaagt catgaatgtg atacaactct atggcatttt
      781 cagagaaatc aaacagtaca aaaagagtac acatattcta aacatggaat gcacttcaca
      841 cataatatgt ttcctgtacc taacaatttg catatggcac agaatgcctg tgaatgtaat
      901 aaagatgaaa cgctgtgtca tcaatcatcc cttaagaaac aaggacaaac tcatactgaa
      961 aagaaacatg aatgcaatca atgtggaaaa gccttcaaaa ggatttctaa tcttacttta
     1021 tacaagaaaa gtcacatggg tgagaaacaa tatgaatgta aagaatgtgg taaagtgttc
     1081 aatgattcct caaccttaag gagacatgta agaactcaca ctggagagaa accctatgaa
     1141 tgtaatcaat gtggaaaagc tttcagtcaa aaaacatccc ttaaggctca catgagaact
     1201 cacactggag agaaacctta tgaatgtaat cagtgtggaa aatcctttgg cacaagctct
     1261 taccttatag tgcacaagag aatacacact ggagaaaaac tctatgaatg cagtgagtgt
     1321 ggaaaagcct tcaacacgag ctctcacctt aaagttcata agaaaattca taccggagag
     1381 aatgtttatg aatgcagtga ctgtgggaaa gttttcagtg gggtctcatc ccttagaatg
     1441 catataagaa ctcacactgg agagaaaccc tatgaatgta aggaatgtag gaaagccttc
     1501 agtgtttcct cttcccttag gaggcatgtg agaattcaca ctggagagaa accctatgaa
     1561 tgtattcaat gtggaaaagc cttcagtcag agctcttcac ttattataca caagagaatt
     1621 catactgaga gagaaaccct gtagtaatga actagtccgt aacttgaaag tatttcgatt
     1681 tcttggcttt atatatcttg tggaaaggac gaagcccaca aacatgtacc acgatg
//
