LOCUS       sequence-281822-        1952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0601
FEATURES             Location/Qualifiers
     source          1..1952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggactgtt gacatttagg gatgtggcca tagaattctc tctggaggag
       61 tggcagtgcc tggacactgc acagaagaat ttatatagga atgtgatgtt agagaactac
      121 agaaacctgg ccttcctggg tattgctgtc tctaagccag acctcatcat ctgtctggag
      181 aaagaaaaag agccctggaa tatgaagcga gatgagatgg tggatgaacc cccaggtata
      241 tgtcctcatt ttgctcaaga catttggcca gagcagggcg tggaagattc ttttcaaaaa
      301 gtaatactaa gaagatttga aaaatgtgga catgagaatt tacagttaag aaaaggttgt
      361 aaaagtgtgg atgagtgtaa ggtacacaaa gaaggttata atggacttaa ccagtgtttc
      421 acaactaccc agggcaaagc ttctcaatgt ggtaaatatt tgaaagtctt ttataaattt
      481 ataaatttaa acagatataa gataagacat actagaaaga aacctttcaa atgtaaaaat
      541 tgtgtcaaat cattttgcat gttttcacac aaaacccagc ataaaagcat atatactaca
      601 gagaagtcct acaaatgtaa agaatgtgga aaaaccttta attggtcctc aacccttact
      661 aatcataaga aaactcatac tgaagaaaag ccctacaaat gtgaagaata tggcaaagct
      721 tttaatcaat cctcaaatta tactacacat aaggtaactc atactggaga gaagccttac
      781 aagtgtgaag aatgtggcaa agcatttagc caatcctcaa cactaaccat acataagagg
      841 atacatactg gagagaaacc ctgcaaatgt gaagaatgtg gcaaagcatt tagccaaccc
      901 tcagcactaa ccatacataa gaggatgcac attggagaga aaccctacaa atgtgaagaa
      961 tgtggcaaag catttgtttg gtcctcaacc ctaactagac ataagaggct gcacagtgga
     1021 gagaaaccct acaaatgtga agaatgtgcc aaagctttta gccaattcgg acaccttact
     1081 acacatagga taattcatac tggagagaaa ccctacaaat gtgaagaatg tggcaaagca
     1141 tttatatggc cctcaaccct aactaaacat aagaggattc acactggaga gaaaccctac
     1201 aaatgtgaag aatgtggcaa agcttttcat cgatcctcaa atcttactaa acataagata
     1261 attcatactg gagagaaacc ttacaagtgt gaagaatgcg gcaaagcgtt tatatggtcc
     1321 tcaaacctta ctgaacataa gaaaattcat actagagaga aaccctacaa atgtgaagaa
     1381 tgtagtaaag catttagccg atcctcagcc ctaactacac ataagaggat gcacactgga
     1441 gagaaaccct acaaatgtga agaatgtggc aaagctttta gccagtcctc aacccttact
     1501 gcacataaga taattcatac tggagagaaa ccctacaaat gtgaagaatg tggcaaagca
     1561 tttatattgt cctcgaccct atctaaacat aagaggattc acactggaga gaaaccctac
     1621 aaatgtgaag aatgtggcaa aacttttaat caatcctcaa atcttagtac acataagata
     1681 attcatactg gagagaaacc ttacaagtgt gaagaatgtg gaaaagcgtt taatcgatcc
     1741 tcaaatctta gtacgcataa gataattcat actggagaga aaccttacaa gtgtgacgaa
     1801 tgtggcaaat catttatctg gtcctcaacc ctttttaagc ataagaggat tcatacttag
     1861 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1921 aaggacgaca taagtcaacc atcttcactc cc
//
