LOCUS       sequence-281828-        1604 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0611
FEATURES             Location/Qualifiers
     source          1..1604
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaccacac ggagggctcg cccgcggagg agccgcctgc gcatgctcca
       61 tcgcctggga aatttggtga gcggcctcca cctaaacgac ttactaggga agctatgcga
      121 aattatttaa aagagcgagg ggatcaaaca gtacttattc ttcatgcaaa agttgcacag
      181 aagtcatatg gaaatgaaaa aaggtttttt tgcccacctc cttgtgtata tcttatgggc
      241 agtggatgga agaaaaaaaa agaacaaatg gaacgcgatg gttgttctga acaagagtct
      301 caaccgtgtg catttattgg gataggaaat agtgaccaag aaatgcagca gctaaacttg
      361 gaaggaaaga actattgcac agccaaaaca ttgtatatat ctgactcaga caagcgaaag
      421 cacttcatgt tgtctgtaaa gatgttctat ggcaacagtg atgacattgg tgtgttcctc
      481 agcaagcgga taaaagtcat ctccaaacct tccaaaaaga agcagtcatt gaaaaatgct
      541 gacttatgca ttgcctcagg aacaaaggtg gctctgttta atcgactacg atcccagaca
      601 gttagtacca gatacttgca tgtagaagga ggtaattttc atgccagttc acagcagtgg
      661 ggagcctttt ttattcatct cttggatgat gatgaatcag aaggagaaga attcacagtc
      721 cgagatggct acatccatta tggacaaaca gtcaaacttg tgtgctcagt tactggcatg
      781 gcactcccaa gattgataat taggaaagtt gataagcaga ccgcattatt ggatgcagat
      841 gatcctgtgt cacaactcca taaatgtgca ttttacctta aggatacaga aagaatgtat
      901 ttgtgccttt ctcaagaaag aataattcaa tttcaggcca ctccatgtcc aaaagaacca
      961 aataaagaga tgataaatga tggcgcttcc tggacaatca ttagcacaga taaggcagag
     1021 tatacatttt atgagggaat gggccctgtc cttgccccag tcactcctgt gcctgtggta
     1081 gagagccttc agttgaatgg cggtggggac gtagcaatgc ttgaacttac aggacagaat
     1141 ttcactccaa atttacgagt gtggtttggg gatgtagaag ctgaaactat gtacaggtgt
     1201 ggagagagta tgctctgtgt cgtcccagac atttctgcat tccgagaagg ttggagatgg
     1261 gtccggcaac cagtccaggt tccagtaact ttggtccgaa atgatggaat catttattcc
     1321 accagcctta cctttaccta cacaccagaa ccagggccgc ggccacattg cagtgcagca
     1381 ggagcaatcc ttcgagccaa ttcaagccag gtgcccccta acgaatcaaa cacaaacagc
     1441 gagggaagtt acacaaacgc cagcacaaat tcaaccagtg tcacatcatc tacagccaca
     1501 gtggtatcct agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1561 atatcttgtg gaaaggacga cgtagcctat acgaacttca acaa
//
