LOCUS       sequence-281831-        1730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0627
FEATURES             Location/Qualifiers
     source          1..1730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccgcggg agccgggacg gcgggccccg cttccggccc gggcgtcgtg
       61 cgtgacccag cggcgtcaca gccgaggaag cggcccggcc gggagggcgg ggagggcgcg
      121 cggcgatcgg acacgatggc gggaggaggc gggagtagcg acggcagcgg gcgggcagct
      181 ggcaggcggg cgtcccgcag tagcgggcgg gcccggcggg ggcgccacga gccggggctg
      241 gggggcccgg cggagcgcgg cgcgggggag gcacggctgg aagaggcagt caatcgctgg
      301 gtgctcaagt tctacttcca cgaggcgctg cgggcctttc ggggtagccg gtacggggac
      361 ttcagacaga tccgggacat catgcaggct ttgcttgtca ggcccttggg gaaggagcac
      421 accgtgtccc gattgctgcg ggttatgcag tgtctgtcgc ggattgaaga aggggaaaat
      481 ttagactgtt cctttgatat ggaggctgag ctcacaccac tggaatcagc tatcaatgtg
      541 ctggagatga ttaaaacgga atttacactg acagaagcag tggtcgaatc cagtagaaaa
      601 ctggtcaagg aagctgctgt cattatttgt atcaaaaaca aagaatttga aaaggcttca
      661 aaaattttga aaaaacatat gtccaaggac cccacaactc agaagctgag aaatgatctc
      721 ctgaatatta ttcgagaaaa gaacttggcc catcctgtta tccagaactt ttcatatgag
      781 accttccagc agaagatgct gcgcttcctg gagagccacc tggatgacgc cgagccctac
      841 ctcctcacga tggccaaaaa ggctttgaaa tctgagtccg ctgcctcaag tacagggaag
      901 gaagataaac agccagcacc agggcctgtg gaaaagccac ccagagaacc cgcaaggcag
      961 ctacggaatc ctccaaccac cattggaatg atgactctga aagcagcttt caagactctg
     1021 tctggtgcac aggattctga ggcagccttt gcaaaactgg accagaagga tctggttctt
     1081 cctactcaag ctctcccagc atcaccagcc ctcaaaaaca agagacccag aaaagatgaa
     1141 aacgaaagtt cagccccggc tgacggtgag ggtggctcgg aactgcagcc caagaacaag
     1201 cgcatgacaa taagcagatt ggtcttggag gaggacagcc agagtactga gcccagcgca
     1261 ggcctcaact cctcccagga ggccgcttca gcgccaccat ccaagcccac cgttctcaac
     1321 caacccctcc ctggagagaa gaatcccaaa gtacccaaag gcaagtggaa cagctctaat
     1381 ggggttgaag aaaaggagac ttgggtggaa gaggatgaac tgtttcaagt tcaggcagca
     1441 ccagatgaag acagtacaac caatataaca aaaaagcaga agtggactgt agaagaaagc
     1501 gagtgggtca aggctggagt gcagaaatat ggggaaggaa actgggctgc catttctaaa
     1561 aattacccat ttgttaaccg aacagctgtg atgattaagg atcgctggcg gaccatgaaa
     1621 agacttggca tgaactagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1681 ctttatatat cttgtggaaa ggacgaatca tggcggtcgg tacccaggtt
//
