LOCUS       sequence-281837-        5966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5966)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 5966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0659
FEATURES             Location/Qualifiers
     source          1..5966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 accgccacca tgcggggccc agtgggcacc gaggaggagc tgccgcggct gttcgccgag
       61 gagatggaga atgaggacga gatgtcagaa gaagaagatg gtggtcttga agccttcgat
      121 gactttttcc ctgtggagcc cgtgagcctt cctaagaaga agaaacccaa gaagctcaag
      181 gaaaacaagt gtaaagggaa gcggaagaag aaagagggga gcaatgatga gctatcagag
      241 aatgaagagg atctggaaga gaagtcggag agtgaaggca gtgactactc cccgaataaa
      301 aagaagaaga agaaactcaa ggacaagaag gagaaaaaag ccaagcgaaa aaagaaggat
      361 gaggatgagg atgataatga tgatggatgc ttaaaggagc ccaagtcctc ggggcagctc
      421 atggccgagt ggggcctgga cgacgtggac tacctgttct cggaggagga ttaccacacg
      481 ctgaccaact acaaggcctt cagccagttc ctcaggccac tcattgccaa gaagaacccg
      541 aagatcccca tgtccaaaat gatgaccgtc ctgggtgcca agtggcggga gttcagcgcc
      601 aacaacccct tcaagggcag ctccgcggca gcagcggcgg cggcggtggc tgcggctgta
      661 gagacggtca ccatctcccc tccgctagcc gtcagccccc cgcaggtgcc ccagcctgtg
      721 cctatccgca aggccaagac caaggagggc aaagggcctg gagtgaggaa gaagatcaaa
      781 ggctccaaag atgggaagaa aaagggcaaa gggaaaaaga cggccgggct caagttccgc
      841 ttcgggggga tcagcaacaa gaggaagaaa ggctcctcga gtgaagaaga tgagagggag
      901 gagtcggact tcgacagcgc cagcatccac agtgcctccg tgcgctccga atgctctgca
      961 gccctgggca agaagagcaa gaggaggcgc aagaagaaga ggattgatga tggtgacggc
     1021 tatgagacag accaccagga ttactgtgag gtgtgccagc agggtgggga gatcatcctg
     1081 tgcgacacct gcccgagggc ctaccatctc gtatgcctgg acccagagct ggagaaggct
     1141 cccgagggca agtggagctg cccccactgt gagaaggagg ggatccagtg ggagccgaag
     1201 gacgacgacg atgaagagga ggagggcggc tgcgaggagg aggaggacga ccacatggag
     1261 ttctgccgcg tgtgcaagga cgggggcgag ctgctctgct gcgacgcctg cccctcctcc
     1321 taccacctgc attgcctcaa cccgccgctg cccgagatcc caaacggtga atggctctgc
     1381 ccgcgctgta cttgcccccc actgaagggc aaagtccagc ggattctaca ctggaggtgg
     1441 acggagcccc ctgccccctt catggtgggg ctgccggggc ctgacgtgga gcccagcctc
     1501 cctccaccta agcccctgga gggcatccct gagagagagt tctttgtcaa gtgggcaggg
     1561 ctgtcctact ggcattgctc ctgggtgaag gagctacagc tggagctgta ccacacggtg
     1621 atgtatcgca actaccaaag aaagaacgac atggatgagc cgcccccctt tgactacggc
     1681 tctggggatg aagacggcaa gagcgagaag aggaagaaca aggaccccct ctatgccaag
     1741 atggaggagc gcttctaccg ctatggcatc aagccagagt ggatgatgat tcaccgaatc
     1801 ctgaaccata gctttgacaa gaagggggat gtgcactacc tgatcaagtg gaaagacctg
     1861 ccctacgacc agtgcacctg ggagatcgat gacatcgaca tcccctacta cgacaacctc
     1921 aagcaggcct actggggcca cagggagctg atgctgggag aagacaccag gctgcccaag
     1981 aggctgctca agaagggcaa gaagctgagg gacgacaagc aggagaagcc gccggacacg
     2041 cccattgtgg accccacggt caagttcgac aagcagccat ggtacatcga ctccacaggc
     2101 ggcacactgc acccgtacca gctggagggc ctcaactggc tgcgcttctc ttgggcccag
     2161 ggcactgaca ccatcctggc cgatgagatg ggtctgggca agacggtgca gaccatcgtg
     2221 ttcctttact ccctctacaa ggagggccac tccaaagggc cctacctggt tagcgcgccc
     2281 ctctccacca tcatcaactg ggaacgcgag tttgagatgt gggcgcccga cttctacgtg
     2341 gtcacctaca cgggggacaa ggagagccgc tcggtgattc gggagaacga gttttccttt
     2401 gaggacaacg ccattcggag tgggaagaag gtattccgta tgaagaaaga agtgcagatc
     2461 aaattccacg tgctgctcac ctcctatgag ctcatcacca ttgaccaggc catcctgggc
     2521 tccatcgagt gggcctgcct ggtggtagat gaggcccacc gcctcaagaa caaccagtcc
     2581 aagtttttta gggtcttaaa cagctacaag attgattaca agctgctgct gacagggacc
     2641 ccccttcaga acaacctgga ggagctgttc catctcctca acttcctgac tccagagagg
     2701 ttcaacaacc tggagggctt cctggaggag tttgctgaca tctccaagga agaccagatc
     2761 aagaagctgc atgacctgct ggggccgcac atgctcaggc ggctcaaggc tgacgtgttc
     2821 aagaacatgc cggccaagac cgagctcatt gtccgggtgg agctgagcca gatgcagaag
     2881 aagtactaca agttcatcct cacacggaac tttgaggcac tgaactccaa ggggggcggg
     2941 aaccaagtat cgctgctcaa catcatgatg gacctgaaaa agtgctgcaa ccacccctac
     3001 ctcttccctg tggctgccgt ggaggcccct gtcttgccca atggctccta cgatggaagc
     3061 tccctggtca agtcttcagg gaagctcatg ctgctacaga agatgctgaa gaaactgcgg
     3121 gatgaggggc accgtgtgct catcttctcc cagatgacca agatgctgga cctcctggag
     3181 gacttcctgg agtacgaagg ctacaagtat gagcggattg atggtggcat caccgggggc
     3241 ctccggcagg aggcaatcga cagattcaat gcccccgggg cccagcagtt ctgcttcctc
     3301 ctctcaaccc gggcaggtgg tctgggcatc aacctggcca cggcggacac tgtcatcatc
     3361 tacgactcgg actggaaccc gcacaatgac atccaggcct tcagccgcgc ccaccgcatc
     3421 ggccagaaca agaaggtgat gatctaccgc ttcgtgactc gggcctcggt ggaggagcgc
     3481 atcacgcagg tggccaagcg caagatgatg ctcacccacc tggtggtgcg gcccggcctc
     3541 ggctccaagt cggggtccat gaccaagcag gagctggacg acatcctcaa gttcggcacg
     3601 gaggaactct tcaaggacga cgtggagggc atgatgtctc agggccagag gccggtcaca
     3661 cccatccctg atgtccagtc ctccaaaggg gggaacttgg ccgccagtgc aaagaagaag
     3721 cacggtagca ccccgccagg tgacaacaag gacgtggagg acagcagtgt gatccactat
     3781 gacgatgcgg ccatctccaa gctgctggac cggaaccagg acgctacaga tgacacggag
     3841 ctacagaaca tgaacgagta cctgagctcc ttcaaggtgg cgcagtacgt ggtgcgcgag
     3901 gaggacggcg tggaggaggt ggagcgggaa atcatcaagc aggaggagaa cgtggacccc
     3961 gactactggg agaagctgct gcggcaccac tatgagcagc agcaggagga cctggcccgc
     4021 aacctgggca agggcaagcg catccgcaag caggtcaact acaacgatgc ctcccaggag
     4081 gaccaggagt ggcaggatga gctctctgat aaccagtcag aatattccat tggctctgag
     4141 gatgaggatg aggactttga agagaggccg gaagggcaga gtggacgacg acaatcccgg
     4201 aggcagctga agagtgacag ggacaagccc ctgcccccgc ttctcgcccg agttggtggc
     4261 aacatcgagg tgctgggctt caatgcccga cagcggaagg cctttctgaa cgccatcatg
     4321 cgctggggca tgcccccgca ggacgccttc aactcccact ggctggtgcg ggaccttcga
     4381 gggaagagcg agaaggagtt tagagcctat gtgtccctct tcatgcggca cctgtgtgag
     4441 ccgggggcgg atggtgcaga gaccttcgca gacggcgtgc cccgggaggg cctctccagg
     4501 cagcacgtgc tgacccgcat cggggtcatg tcactagtta ggaagaaggt tcaggagttt
     4561 gagcatgtca acgggaagta cagcacccca gacttgatcc ctgaggggcc cgaggggaag
     4621 aagtcgggcg aggtgatctc ctcggacccc aacacaccag tgcccgccag ccctgcccac
     4681 ctcctgccag ccccgctggg cctgccagac aaaatggaag cccagctggg ctacatggat
     4741 gagaaagacc ccggggcaca gaagccaagg cagcccctgg aagtccaggc ccttccagcc
     4801 gccttggata gagtggagag tgaggacaag cacgagagcc cagccagcaa ggagagagcc
     4861 cgagaggagc ggccagagga gacggagaag gccccgccct ccccggagca gctgccgaga
     4921 gaggaggtgc ttcctgagaa ggagaagatc ctggacaagc tggagctgag cttgatccac
     4981 agcagagggg acagttccga actcaggcca gatgacacca aggctgagga gaaggagccc
     5041 attgaaacac agcaaaatgg tgacaaagag gaagatgacg aggggaagaa ggaggacaag
     5101 aaggggaaat tcaagttcat gttcaacatc gcggacgggg gcttcacgga gttgcacacg
     5161 ctgtggcaga acgaggagcg ggctgctgta tcctctggga aaatctacga catctggcac
     5221 cggcgccatg actactggct gctggcgggc atcgtgacgc acggctacgc ccgctggcag
     5281 gacatccaga atgacccacg gtacatgatc ctcaacgagc ccttcaagtc tgaggtccac
     5341 aagggcaact acctggagat gaagaacaag ttcctggccc gcaggtttaa gctgctggag
     5401 caggcgttgg tcattgagga gcagctccgg agggccgcgt acctgaacat gacgcaggac
     5461 cccaaccacc ccgccatggc cctcaacgcc cgcctggctg aagtggagtg cctcgccgag
     5521 agccaccagc acctgtccaa ggagtccctt gctgggaaca agcctgccaa tgccgtcctg
     5581 cacaaggtcc tgaaccagct ggaggagctg ctgagcgaca tgaaggccga cgtgacccgg
     5641 ctgccatcca tgctgtcccg catccccccg gtggccgccc ggctgcagat gtcggagcgc
     5701 agcatcctga gccgcctgac caaccgcgcc ggggacccca ccatccagca gggcgctttc
     5761 ggctcctccc agatgtacag caacaacttt gggcccaact tccggggccc tggaccggga
     5821 gggattgtca actacaacca gatgcccctg gggccctatg tgaccgatat ctagtaatga
     5881 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     5941 gaggtattaa tttggttgca catcaa
//
