LOCUS       sequence-281852-        2717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0911
FEATURES             Location/Qualifiers
     source          1..2717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggtcgctc caattctaga tcacattctt caaggtcaaa gtctagatca
       61 cagtctagtt ctcgatcaag atcaagatct cattctagaa agaagcgata cagttctagg
      121 tctcgttcca gaacatattc aaggtctcgt agtagagatc gtatgtattc tagagattat
      181 cgtcgcgatt acagaaataa tagaggaatg agacgacctt atgggtacag aggaaggggt
      241 agagggtatt atcaaggagg aggaggtaga tatcatcgag gtggttatag acctgtctgg
      301 aatagaaggc actctaggag tcctagacga ggtcgttcac gttccaggag tccaaaaaga
      361 agatccgttt cttctcaaag atccagaagc agatctcgcc ggtcatatag atcttctagg
      421 tctccaagat catcctcttc tcgttcttca tccccatata gcaaatctcc tgtttctaaa
      481 agacgagggt ctcaggaaaa acaaaccaaa aaagctgaag gggaacccca agaagagagt
      541 ccgttgaaaa gtaaatcaca ggaggaaccg aaagatacat ttgaacatga cccatctgag
      601 tctatcgatg aatttaataa gtcatcagcc acatccggtg atatttggcc tggcctttca
      661 gcttatgata atagtcctag atcaccccat agtccttcac ctattgctac accacctagt
      721 cagagttcat cttgctctga tgctcccatg ctcagtacag ttcactctgc aaaaaatact
      781 ccttctcagc attcacattc cattcagcat agtcctgaaa ggtctgggtc tggttctgtt
      841 ggaaatggat ctagtcgata cagtccttct cagaatagtc caattcatca catcccttca
      901 cgaagaagtc ctgcaaagac aatcgcacca cagaatgctc caagagatga gtctaggggc
      961 cgttcctcgt tttatcctga tggtggagat caggaaactg caaagactgg gaagttctta
     1021 aaaaggttca cagatgaaga gtctagagta ttcctgcttg ataggggtaa taccagggat
     1081 aaagaggctt caaaagagaa aggatcagag aaagggaggg cagagggaga atgggaagat
     1141 caggaagctc tagattactt cagtgataaa gagtctggaa aacaaaagtt taatgattca
     1201 gaaggggatg acacagagga gacagaggat tatagacagt tcaggaagtc agtcctcgca
     1261 gatcagggta aaagttttgc tactgcatct caccggaata ctgaggagga aggactcaag
     1321 tacaagtcca aagtttcact gaaaggcaat agagaaagtg atggatttag agaagaaaaa
     1381 aattataaac ttaaagagac tggatatgta gtggaaaggc ctagcactac aaaagataag
     1441 cacaaagaag aagacaaaaa ttctgaaaga ataacagtaa agaaagaaac tcagtcacct
     1501 gagcaggtaa agtctgaaaa gctcaaagac ctctttgatt acagtccccc tctacacaag
     1561 aatctggatg cacgagaaaa gtctaccttc agagaggaaa gcccacttag gatcaaaatg
     1621 atagcgagtg attctcaccg tcctgaagtc aaactcaaaa tggcacctgt tcctcttgat
     1681 gattctaaca gacctgcttc cttgactaaa gacaggctgc ttgctagtac acttgtccat
     1741 tctgtcaaga aggagcaaga attccgatcc atctttgacc acattaagtt gccacaggcc
     1801 agcaaaagca cttcagagtc atttattcaa cacattgtgt ccttggttca tcatgttaaa
     1861 gagcaatact tcaagtcagc tgcaatgacc ctaaacgagc ggttcacttc gtatcagaaa
     1921 gccactgaag aacatagtac tcggcaaaag agccctgaaa tacacaggag aattgacatc
     1981 tcaccaagta ccctgaggaa gcatacccgt ttagcagggg aagagagagt ttttaaagaa
     2041 gaaaatcaaa agggagataa aaaattaagg tgtgactctg ctgaccttcg gcatgacatt
     2101 gatcgccgta gaaaagaaag aagtaaagaa cggggagatt ccaagggctc cagggaatcc
     2161 agtggatcaa gaaagcagga aaaaactcca aaagattaca aggaatacaa atcttacaaa
     2221 gatgacagta aacataaaag agagcaagat cattctcgat cttcatcctc ttcagcatca
     2281 ccttcttctc ccagttctcg agaagaaaag gagagtaaga aggaaagaga agaagaattt
     2341 aaaactcacc atgaaatgaa agaatactca ggctttgcag gagttagccg accacgagga
     2401 acctttcatg acgacagaga tgatggtgtg gattattggg ccaaaagagg aagaggtcgt
     2461 ggtacttttc aacgtggcag agggcgcttt aacttcaaaa aatcaggtag cagtcctaaa
     2521 tggactcatg acaaatacca aggggatggg attgttgaag atgaagaaga gaccatggaa
     2581 aataatgaag aaaagaagga cagacgcaag gaagaaaagg aatagtaatg aactagtccg
     2641 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgacccttgc
     2701 tccctgaggt acaagcc
//
