LOCUS       sequence-281855-        4424 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4424)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0920
FEATURES             Location/Qualifiers
     source          1..4424
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 accgccacca tgagtggcct cggggacagt tcatccgacc ctgctaaccc agactcacat
       61 aagaggaaag gatcgccatg tgacacactg gcatcaagca cggaaaagag gcgcagggag
      121 caagaaaata aatatttaga agaactagct gagttactgt ctgccaacat tagtgacatt
      181 gacagcttga gtgtaaaacc agacaaatgc aagattttga agaaaacagt cgatcagata
      241 cagctaatga agagaatgga acaagagaaa tcaacaactg atgacgatgt acagaaatca
      301 gacatctcat caagtagtca aggagtgata gaaaaggaat ccttgggacc tcttcttttg
      361 gaggctttgg atggattttt ctttgttgtg aactgtgaag ggagaattgt atttgtgtca
      421 gagaatgtaa ccagctactt aggttacaat caggaggaat taatgaatac gagcgtctac
      481 agcatactgc acgtggggga tcatgcagaa tttgtgaaga atctgctacc aaaatcacta
      541 gtaaatggag ttccttggcc tcaagaggca acacgacgaa atagccatac ctttaactgc
      601 aggatgctaa ttcaccctcc agatgagcca gggaccgaga accaagaagc ttgccagcgt
      661 tatgaagtaa tgcagtgttt cactgtgtca cagccaaaat caattcaaga ggatggagaa
      721 gatttccagt catgtctgat ttgtattgca cggcgattac ctcggcctcc agctattacg
      781 ggtgtagaat cctttatgac caagcaagat actacaggta aaatcatctc tattgatact
      841 agttccctga gagctgctgg cagaactggt tgggaagatt tagtgaggaa gtgcatttat
      901 gcttttttcc aacctcaggg cagagaacca tcttatgcca gacagctgtt ccaagaagtg
      961 atgactcgtg gcactgcctc cagcccctcc tatagattca tattgaatga tgggacaatg
     1021 cttagcgccc acaccaagtg taaactttgc taccctcaaa gtccagacat gcaacctttc
     1081 atcatgggaa ttcatatcat cgacagggag cacagtgggc tttctcctca agatgacact
     1141 aattctggaa tgtcaattcc ccgagtaaat ccctcggtca atcctagtat ctctccagct
     1201 catggtgtgg ctcgttcatc cacattgcca ccatccaaca gcaacatggt atccaccaga
     1261 ataaaccgcc agcagagctc agaccttcat agcagcagtc atagtaattc tagcaacagc
     1321 caaggaagtt tcggatgctc acccggaagt cagattgtag ccaatgttgc cttaaaccaa
     1381 ggacaggcca gttcacagag cagtaatccc tctttaaacc tcaataattc tcctatggaa
     1441 ggtacaggaa tatccctagc acagttcatg tctccaagga gacaggttac ttctggattg
     1501 gcaacaaggc ccaggatgcc aaacaattcc tttcctccta atatttcgac attaagctct
     1561 cccgttggca tgacaagtag tgcctgtaat aataataacc gatcttattc aaacatccca
     1621 gtaacatctt tacagggtat gaatgaagga cccaataact ccgttggctt ctctgccagt
     1681 tctccagtcc tcaggcagat gagctcacag aattcaccta gcagattaaa tatacaacca
     1741 gcaaaagctg agtccaaaga taacaaagag attgcctcaa ttttaaatga aatgattcaa
     1801 tctgacaaca gctctagtga tggcaaacct ctggattcag ggcttctgca taacaatgac
     1861 agactttcag atggagacag taaatactct caaaccagtc acaaactagt gcagcttttg
     1921 acaacaactg ccgaacagca gttacggcat gctgatatag acacaagctg caaagatgtc
     1981 ctgtcttgca caggcacttc caactctgcc tctgctaact cttcaggagg ttcttgtccc
     2041 tcttctcata gctcattgac agaacggcat aaaattctac accggctctt acaggagggt
     2101 agcccctcag atatcaccac tttgtctgtc gagcctgata aaaaggacag tgcatctact
     2161 tctgtgtcag tgactggaca ggtacaagga aactccagta taaaactaga actggatgct
     2221 tcaaagaaaa aagaatcaaa agaccatcag ctcctacgct atcttttaga taaagatgag
     2281 aaagatttaa gatcaactcc aaacctgagc ctggatgatg taaaggtgaa agtggaaaag
     2341 aaagaacaga tggatccatg taatacaaac ccaaccccaa tgaccaaacc cactcctgag
     2401 gaaataaaac tggaggccca gagccagttt acagctgacc ttgaccagtt tgatcagtta
     2461 ctgcccacgc tggagaaggc agcacagttg ccaggcttat gtgagacaga caggatggat
     2521 ggtgcggtca ccagtgtaac catcaaatcg gagatcctgc cagcttcact tcagtccgcc
     2581 actgccagac ccacttccag gctaaataga ttacctgagc tggaattgga agcaattgat
     2641 aaccaatttg gacaaccagg aacaggcgat cagattccat ggacaaataa tacagtgaca
     2701 gctataaatc agagtaaatc agaagaccag tgtattagct cacaattaga tgagcttctc
     2761 tgtccaccca caacagtaga agggagaaat gatgagaagg ctcttcttga acagctggta
     2821 tccttcctta gtggcaaaga tgaaactgag ctagctgaac tagacagagc tctgggaatt
     2881 gacaaacttg ttcagggggg tggattagat gtattatcag agagatttcc accacaacaa
     2941 gcaacgccac ctttgatcat ggaagaaaga cccaaccttt attcccagcc ttactcttct
     3001 ccttctccta ctgccaatct ccctagccct ttccaaggca tggtcaggca aaaaccttca
     3061 ctggggacga tgcctgttca agtaacacct ccccgaggtg ctttttcacc tggcatgggc
     3121 atgcagccca ggcaaactct aaacagacct ccggctgcac ctaaccagct tcgacttcaa
     3181 ctacagcagc gattacaggg acaacagcag ttgatacacc aaaatcggca agctatctta
     3241 aaccagtttg cagcaactgc tcctgttggc atcaatatga gatcaggcat gcaacagcaa
     3301 attacacctc agccacccct gaatgctcaa atgttggcac aacgtcagcg ggaactgtac
     3361 agtcaacagc accgacagag gcagctaata cagcagcaaa gagccatgct tatgaggcag
     3421 caaagctttg ggaacaacct ccctccctca tctggactac cagttcaaat ggggaacccc
     3481 cgtcttcctc agggtgctcc acagcaattc ccctatccac caaactatgg tacaaatcca
     3541 ggaaccccac ctgcttctac cagcccgttt tcacaactag cagcaaatcc tgaagcatcc
     3601 ttggccaacc gcaacagcat ggtgagcaga ggcatgacag gaaacatagg aggacagttt
     3661 ggcactggaa tcaatcctca gatgcagcag aatgtcttcc agtatccagg agcaggaatg
     3721 gttccccaag gtgaggccaa ctttgctcca tctctaagcc ctgggagctc catggtgccg
     3781 atgccaatcc ctcctcctca gagttctctt ctccagcaaa ctccacctgc ctccgggtat
     3841 cagtcaccag acatgaaggc ctggcagcaa ggagcgatag gaaacaacaa tgtgttcagt
     3901 caagctgtcc agaaccagcc cacgcctgca cagccaggag tatacaacaa catgagcatc
     3961 accgtttcca tggcaggtgg aaatacgaat gttcagaaca tgaacccaat gatggcccag
     4021 atgcagatga gctctttgca gatgccagga atgaacactg tgtgccctga gcagataaat
     4081 gatcccgcac tgagacacac aggcctctac tgcaaccagc tctcatccac tgaccttctc
     4141 aaaacagaag cagatggaac ccaggtgcaa caggttcagg tgtttgctga cgtccagtgt
     4201 acagtgaatc tggtaggcgg ggacccttac ctgaaccagc ctggtccact gggaactcaa
     4261 aagcccacgt caggaccaca gaccccccag gcccagcaga agagcctcct tcagcagcta
     4321 ctgactgaat agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     4381 atatcttgtg gaaaggacga aacaattcac cgtatcgtct cgga
//
