LOCUS       sequence-281861-         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0927
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctcccaa gaaacgccca gaaacccaga agacctccga gattgtatta
       61 cgccccagga acaagaggag caggagtccc ctggagctgg agcccgaggc caagaagctc
      121 tgtgcgaagg gctccggtcc tagcagaaga tgtgactcag actgcctctg ggtggggctg
      181 gctggcccac agatcctgcc accatgccgc agcatcgtca ggaccctcca ccagcataag
      241 ctgggcagag cttcctggcc atctgtccag caggggctcc agcagtcctt tttgcacact
      301 ctggattctt accggatatt acaaaaggct gccccctttg acaggagggc tacatccttg
      361 gcgtggcacc caactcaccc cagcaccgtg gctgtgggtt ccaaaggggg agatatcatg
      421 ctctggaatt ttggcatcaa ggacaaaccc accttcatca aaggggcagc ctggcatcct
      481 cgctacaacc tcattgttgt gggccgatac ccagatccta atttcaaaag ttgtacccct
      541 tatgaattga ggacgatcga cgtgttcgat ggaaactcag ggaagatgat gtgtcagctc
      601 tatgacccag aatcttctgg catcagttcg cttaatgaat tcaatcccat gggggacacg
      661 ctggcctctg caatgggtta ccacattctc atctggagcc aggaggaagc caggacacgg
      721 aagtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
      781 tgtggaaagg acgatgagcc gtgcctcata cacgaata
//
