LOCUS       sequence-281867-        1598 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0931
FEATURES             Location/Qualifiers
     source          1..1598
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1141..1161
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
ORIGIN
        1 accgccacca tggccgccgc cgccgccgcc gcgccgagcg gaggaggagg aggaggcgag
       61 gaggagagac tggaagaaaa gtcagaagac caggacctcc agggcctcaa ggacaaaccc
      121 ctcaagttta aaaaggtgaa gaaagataag aaagaagaga aagagggcaa gcatgagccc
      181 gtgcagccat cagcccacca ctctgctgag cccgcagagg caggcaaagc agagacatca
      241 gaagggtcag gctccgcccc ggctgtgccg gaagcttctg cctcccccaa acagcggcgc
      301 tccatcatcc gtgaccgggg acccatgtat gatgacccca ccctgcctga aggctggaca
      361 cggaagctta agcaaaggaa atctggccgc tctgctggga agtatgatgt gtatttgatc
      421 aatccccagg gaaaagcctt tcgctctaaa gtggagttga ttgcgtactt cgaaaaggta
      481 ggcgacacat ccctggaccc taatgatttt gacttcacgg taactgggag agggagcccc
      541 tcccggcgag agcagaaacc acctaagaag cccaaatctc ccaaagctcc aggaactggc
      601 agaggccggg gacgccccaa agggagcggc accacgagac ccaaggcggc cacgtcagag
      661 ggtgtgcagg tgaaaagggt cctggagaaa agtcctggga agctccttgt caagatgcct
      721 tttcaaactt cgccaggggg caaggctgag gggggtgggg ccaccacatc cacccaggtc
      781 atggtgatca aacgccccgg caggaagcga aaagctgagg ccgaccctca ggccattccc
      841 aagaaacggg gccgaaagcc ggggagtgtg gtggcagccg ctgccgccga ggccaaaaag
      901 aaagccgtga aggagtcttc tatccgatct gtgcaggaga ccgtactccc catcaagaag
      961 cgcaagaccc gggagacggt cagcatcgag gtcaaggaag tggtgaagcc cctgctggtg
     1021 tccaccctcg gtgagaagag cgggaaagga ctgaagacct gtaagagccc tgggcggaaa
     1081 agcaaggaga gcagccccaa ggggcgcagc agcagcgcct cctcaccccc caagaaggag
     1141 caccaccacc atcaccacca ctcagagtcc ccaaaggccc ccgtgccact gctcccaccc
     1201 ctgcccccac ctccacctga gcccgagagc tccgaggacc ccaccagccc ccctgagccc
     1261 caggacttga gcagcagcgt ctgcaaagag gagaagatgc ccagaggagg ctcactggag
     1321 agcgacggct gccccaagga gccagctaag actcagcccg cggttgccac cgccgccacg
     1381 gccgcagaaa agtacaaaca ccgaggggag ggagagcgca aagacattgt ttcatcctcc
     1441 atgccaaggc caaacagaga ggagcctgtg gacagccgga cgcccgtgac cgagagagtt
     1501 agctagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1561 tgtggaaagg acgaagtaga ggctgtcaga cctagcga
//
