LOCUS       sequence-281873-        1889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0934
FEATURES             Location/Qualifiers
     source          1..1889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggaccatt gacatttagg gatgtggcca tagaattctc tctgaaggag
       61 tggcaatgcc tggacactgc acagcggaat ttatatagaa atgtgatgtt agagaactac
      121 agaaacctgg tcttcctggg tattactgtt tctaagccag acctgatcac ttgtctggag
      181 caagggaaag aggcctggag tatgaagaga catgagatca tggtggccaa acccacagtt
      241 atgtgttctc attttgccca agacctttgg ccggagcaga atataaaaga ttctttccaa
      301 aaagtgacac tgaaaagata tggaaaatgt agacatgaaa atttaccatt aagaaaaggc
      361 tgtgaaagta tggatgagtg taagatgcac aaaggaggtt gtaatggact taaccaatgt
      421 ctcacagcta cccagagcaa aatatttcaa tgtgataaat atgtaaaagt cgctcataaa
      481 ttttcaaatt caaacagaca tgagataaga catactaaaa agaaaccttt caaatgtaca
      541 aaatgtggca aatcatttgg catgatttca tgcctaactg aacatagcag aattcatact
      601 agagtaaatt tctacaaatg tgaagaatgt ggaaaagcct ttaactggtc ctcaaccctt
      661 actaaacata agagaattca tacgggagag aaaccttaca aatgtgaaga atgtggtaaa
      721 gcctttaacc agtcctcaaa ccttattaaa cataagaaaa ttcatactgg agagaaaccc
      781 tacaaatgtg aagaatgtgg caaaactttt aaccgattct caactcttac tacccataag
      841 ataattcata ctggagagaa accctacaaa tgtaaagaat gtggtaaagc ttttaaccga
      901 tcttcaaccc ttactaccca tagaaaaatt catactggag agaaacctta caaatgtgaa
      961 gaatgtggca aagcctttaa gcagtcctca aaccttacta cacataagat aattcatact
     1021 ggagagaaac cctacaaatg taaaaaatgt ggaaaagcct ttaaccagtc tgcacacctt
     1081 accacacatg aggtaattca tactggagag aaaccctaca aatgtgaaaa atgtggaaaa
     1141 gcctttaatc atttctcaca ccttactaca cataagataa ttcatactgg agagaaacct
     1201 tacaaatgta aagaatgtgg taaagctttt aaacactctt caacccttac taaacataag
     1261 ataattcata ctggagagaa gccttacaaa tgtaaagaat gtgaaaaagc ttttaaccaa
     1321 tcctcaaaac ttactgaaca taagaaaatt catactggag agaaacccta tgaatgtgaa
     1381 aaatgtggca aagcttttaa ccagtcctca aatcttacta gacataagaa aagtcataca
     1441 gaagagaaac cttacaaatg tgaagaatgt ggcaaaggtt ttaaatggcc ctcaaccctt
     1501 actatccata agataattca tactggagag aaaccataca aatgtgaaga atgtggcaaa
     1561 gcttttaacc aatcctcaaa acttaccaaa cataagaaaa ttcatactgg agagaaaccc
     1621 tacacatgtg aagaatgtgg caaagccttt aaccagtcct caaaccttac taaacataag
     1681 agaattcata ctggagaaaa accttacaaa tgtgaagaat gtgacaaagc ttttaaatgg
     1741 tcctcagtcc ttactaaaca taagataatt cataccggag aaaaattaca aatatagtaa
     1801 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1861 gacgatggcc taaataggcg ctcgcacca
//
