LOCUS       sequence-281876-         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0943
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaagggag cgcggtgact gtccttgagc gcggaggggc gagctcgccg
       61 gcggagcgcc ggagcaagcg gaggcgcagg agcggcggcg acggcggcgg cggcggcggc
      121 gcccgagcac ccgagggggt ccgagccccg gcagccggcc agccccgcgc cacaaaggga
      181 gcgcccccgc cgcccggcac cccgcctccc tccccaatgt cctcggccat cgaaaggaag
      241 agcctggacc cttcagagga accagtggat gaggtgctgc agatcccccc atccctgctg
      301 acatgcggcg gctgccagca gaacattggg gaccgctact tcctgaaggc catcgaccag
      361 tactggcacg aggactgcct gagctgcgac ctctgtggct gccggctggg tgaggtgggg
      421 cggcgcctct actacaaact gggccggaag ctctgccgga gagactatct caggcttttt
      481 gggcaagacg gtctctgcgc atcctgtgac aagcggattc gtgcctatga gatgacaatg
      541 cgggtgaaag acaaagtgta tcacctggaa tgtttcaaat gcgccgcctg tcagaagcat
      601 ttctgtgtag gtgacagata cctcctcatc aactctgaca tagtgtgcga acaggacatc
      661 tacgagtgga ctaagatcaa tgggatgata tagtaatgaa ctagtccgta acttgaaagt
      721 atttcgattt cttggcttta tatatcttgt ggaaaggacg agcttgaaag aatctcaaga
      781 tggac
//
