LOCUS       sequence-281897-        3395 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1001
FEATURES             Location/Qualifiers
     source          1..3395
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2591..2608
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 accgccacca tggccaccca ggtaatgggg cagtcttctg gaggaggagg gctgtttacc
       61 agcagtggca acattggaat ggccctgcct aacgacatgt atgacttgca tgacctttcc
      121 aaagctgaac tggccgcacc tcagcttatt atgctggcaa atgtggcctt aactggggaa
      181 gtaaatggca gctgctgtga ttacctggtc ggtgaagaaa gacagatggc agaactgatg
      241 ccggttgggg ataacaactt ttcagatagt gaagaaggag aaggacttga agagtctgct
      301 gatataaaag gtgaacctca tggactggaa aacatggaac tgagaagttt ggaactcagc
      361 gtcgtagaac ctcagcctgt atttgaggca tcaggtgctc cagatattta cagttcaaat
      421 aaagatcttc cccctgaaac acctggagcg gaggacaaag gcaagagctc gaagaccaaa
      481 ccctttcgct gtaagccatg ccaatatgaa gcagaatctg aagaacagtt tgtgcatcac
      541 atcagagttc acagtgctaa gaaatttttt gtggaagaga gtgcagagaa gcaggcaaaa
      601 gccagggaat ctggctcttc cactgcagaa gagggagatt tctccaaggg ccccattcgc
      661 tgtgaccgct gcggctacaa tactaatcga tatgatcact atacagcaca cctgaaacac
      721 cacaccagag ctggggataa tgagcgagtc tacaagtgta tcatttgcac atacacaaca
      781 gtgagcgagt atcactggag gaaacattta agaaaccatt ttccaaggaa agtatacaca
      841 tgtggaaaat gcaactattt ttcagacaga aaaaacaatt atgttcagca tgttagaact
      901 catacaggag aacgcccata taaatgtgaa ctttgtcctt actcaagttc tcagaagact
      961 catctaacta gacatatgcg tactcattca ggtgagaagc catttaaatg tgatcagtgc
     1021 agttatgtgg cctctaatca acatgaagta acccgccatg caagacaggt tcacaatggg
     1081 cctaaacctc ttaattgccc acactgtgat tacaaaacag cagatagaag caacttcaaa
     1141 aaacatgtag agctacatgt gaacccacgg cagttcaatt gccctgtatg tgactatgca
     1201 gcttccaaga agtgtaatct acagtatcac ttcaaatcta agcatcctac ttgtcctaat
     1261 aaaacaatgg atgtctcaaa agtgaaacta aagaaaacca aaaaacgaga ggctgacttg
     1321 cctgataata ttaccaatga aaaaacagaa atagaacaaa caaaaataaa aggggatgtg
     1381 gctggaaaga aaaatgaaaa gtccgtcaaa gcagagaaaa gagatgtctc aaaagagaaa
     1441 aagccttcta ataatgtgtc agtgatccag gtgactacca gaactcgaaa atcagtaaca
     1501 gaggtgaaag agatggatgt gcatacagga agcaattcag aaaaattcag taaaactaag
     1561 aaaagcaaaa ggaagctgga agttgacagc cattctttac atggtcctgt gaatgatgag
     1621 gaatcttcaa caaaaaagaa aaagaaggta gaaagcaaat ccaaaaataa tagtcaggaa
     1681 gtgccaaagg gtgacagcaa agtggaggag aataaaaagc aaaatacttg catgaaaaaa
     1741 agtacaaaga agaaaactct gaaaaataaa tcaagtaaga aaagcagtaa gcctcctcag
     1801 aaggaacctg ttgagaaggg atctgctcag atggaccctc ctcagatggg gcctgctccc
     1861 acagaggcgg ttcagaaggg gcccgttcag gtggagccgc cacctcccat ggagcatgct
     1921 cagatggagg gtgcccagat acggcctgct cctgacgagc ctgttcagat ggaggtggtt
     1981 caggaggggc ctgctcagaa ggagctgctg cctcccgtgg agcctgctca gatggtgggt
     2041 gcccaaattg tacttgctca catggagctg cctcctccca tggagactgc tcagacggag
     2101 gttgcccaaa tggggcctgc tcccatggaa cctgctcaga tggaggttgc ccaggtagaa
     2161 tctgctccca tgcaggtggt ccagaaggag cctgttcaga tggagctgtc tcctcccatg
     2221 gaggtggtcc agaaggagcc tgttcagata gagctgtctc ctcccatgga ggtggtccag
     2281 aaggaacctg ttaagataga gctgtctcct cccatagagg tggtccagaa ggagcctgtt
     2341 cagatggagt tgtctcctcc catgggggtg gttcagaagg agcctgctca gagggagcca
     2401 cctcctccca gagagcctcc ccttcacatg gagccaattt ccaaaaagcc tcctctccga
     2461 aaagataaaa aggaaaagtc taacatgcag agtgaaaggg cacggaagga gcaagtcctt
     2521 attgaagttg gcttagtgcc tgttaaagat agctggcttc taaaggaaag tgtaagcaca
     2581 gaggatctct caccaccatc accaccactg ccaaaggaaa atttaagaga agaggcatca
     2641 ggagaccaaa aattactcaa cacaggtgaa ggaaataaag aagcccctct tcagaaagta
     2701 ggagcagaag aggcagatga gagcctacct ggtcttgctg ctaatatcaa cgaatctacc
     2761 catatttcat cctctggaca aaacttgaat acgccagagg gtgaaacttt aaatggtaaa
     2821 catcagactg acagtatagt ttgtgaaatg aaaatggaca ctgatcagaa cacaagagag
     2881 aatctcactg gtataaattc aacagttgaa gaaccagttt caccaatgct tcccccttca
     2941 gcagtagaag aacgtgaagc agtgtccaaa actgcactgg catcacctcc tgctacaatg
     3001 gcagcaaatg agtctcagga aattgatgaa gatgaaggca tccacagcca tgaaggaagt
     3061 gacctaagtg acaacatgtc agagggtagt gatgattctg gattgcatgg ggctcggcca
     3121 gttccacaag aatctagcag aaaaaatgca aaggaagcct tggcagtcaa agcggctaag
     3181 ggagattttg tttgtatctt ctgtgatcgt tctttcagaa agggaaaaga ttacagcaaa
     3241 cacctcaatc gccatttggt taatgtgtac tatcttgaag aagcagctca agggcaggag
     3301 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     3361 ggaaaggacg aaaatgcagc attaggtagg gcgga
//
