LOCUS       sequence-281900-        7430 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7430)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 7430)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1108
FEATURES             Location/Qualifiers
     source          1..7430
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4876..4885
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctgagaa cttgctggac ggaccgccca accccaaaag agccaaactc
       61 agctcgcccg gtttctcggc gaatgacagc acagattttg gatcattgtt tgacttggaa
      121 aatgatcttc ctgatgagct gatacccaat ggaggagaat taggcctttt aaacagtggg
      181 aaccttgttc cagatgctgc ttccaaacat aaacaactgt cggagcttct acgaggaggc
      241 agcggctcta gtatcaaccc aggaatagga aatgtgagcg ccagcagccc cgtgcagcag
      301 ggcctgggtg gccaggctca agggcagccg aacagtgcta acatggccag cctcagtgcc
      361 atgggcaaga gccctctgag ccagggagat tcttcagccc ccagcctgcc taaacaggca
      421 gccagcacct ctgggcccac ccccgctgcc tcccaagcac tgaatccgca agcacaaaag
      481 caagtggggc tggcgactag cagccctgcc acgtcacaga ctggacctgg tatctgcatg
      541 aatgctaact ttaaccagac ccacccaggc ctcctcaata gtaactctgg ccatagctta
      601 attaatcagg cttcacaagg gcaggcgcaa gtcatgaatg gatctcttgg ggctgctggc
      661 agaggaaggg gagctggaat gccgtaccct actccagcca tgcagggcgc ctcgagcagc
      721 gtgctggctg agaccctaac gcaggtttcc ccgcaaatga ctggtcacgc gggactgaac
      781 accgcacagg caggaggcat ggccaagatg ggaataactg ggaacacaag tccatttgga
      841 cagcccttta gtcaagctgg agggcagcca atgggagcca ctggagtgaa cccccagtta
      901 gccagcaaac agagcatggt caacagtttg cccaccttcc ctacagatat caagaatact
      961 tcagtcacca acgtgccaaa tatgtctcag atgcaaacat cagtgggaat tgtacccaca
     1021 caagcaattg caacaggccc cactgcagat cctgaaaaac gcaaactgat acagcagcag
     1081 ctggttctac tgcttcatgc tcataagtgt cagagacgag agcaagcaaa cggagaggtt
     1141 cgggcctgct cgctcccgca ttgtcgaacc atgaaaaacg ttttgaatca catgacgcat
     1201 tgtcaggctg ggaaagcctg ccaagttgcc cattgtgcat cttcacgaca aatcatctct
     1261 cattggaaga actgcacacg acatgactgt cctgtttgcc tccctttgaa aaatgccagt
     1321 gacaagcgaa accaacaaac catcctgggg tctccagcta gtggaattca aaacacaatt
     1381 ggttctgttg gcacagggca acagaatgcc acttctttaa gtaacccaaa tcccatagac
     1441 cccagctcca tgcagcgagc ctatgctgct ctcggactcc cctacatgaa ccagccccag
     1501 acgcagctgc agcctcaggt tcctggccag caaccagcac agcctcaaac ccaccagcag
     1561 atgaggactc tcaaccccct gggaaataat ccaatgaaca ttccagcagg aggaataaca
     1621 acagatcagc agcccccaaa cttgatttca gaatcagctc ttccgacttc cctgggggcc
     1681 acaaacccac tgatgaacga tggctccaac tctggtaaca ttggaaccct cagcactata
     1741 ccaacagcag ctcctccttc tagcaccggt gtaaggaaag gctggcacga acatgtcact
     1801 caggacctgc ggagccatct agtgcataaa ctcgtccaag ccatcttccc aacacctgat
     1861 cccgcagctc taaaggatcg ccgcatggaa aacctggtag cctatgctaa gaaagtggaa
     1921 ggggacatgt acgagtctgc caacagcagg gatgaatatt atcacttatt agcagagaaa
     1981 atctacaaga tacaaaaaga actagaagaa aaacggaggt cgcgtttaca taaacaaggc
     2041 atcttgggga accagccagc cttaccagcc ccgggggctc agccccctgt gattccacag
     2101 gcacaacctg tgagacctcc aaatggaccc ctgtccctgc cagtgaatcg catgcaagtt
     2161 tctcaaggga tgaattcatt taaccccatg tccttgggga acgtccagtt gccacaagca
     2221 cccatgggac ctcgtgcagc ctccccaatg aaccactctg tccagatgaa cagcatgggc
     2281 tcagtgccag ggatggccat ttctccttcc cgaatgcctc agcctccgaa catgatgggt
     2341 gcacacacca acaacatgat ggcccaggcg cccgctcaga gccagtttct gccacagaac
     2401 cagttcccgt catccagcgg ggcgatgagt gtgggcatgg ggcagccgcc agcccaaaca
     2461 ggcgtgtcac agggacaggt gcctggtgct gctcttccta accctctcaa catgctgggg
     2521 cctcaggcca gccagctacc ttgccctcca gtgacacagt caccactgca cccaacaccg
     2581 cctcctgctt ccacggctgc tggcatgcca tctctccagc acacgacacc acctgggatg
     2641 actcctcccc agccagcagc tcccactcag ccatcaactc ctgtgtcgtc ttccgggcag
     2701 actcccaccc cgactcctgg ctcagtgccc agtgctaccc aaacccagag cacccctaca
     2761 gtccaggcag cagcccaggc ccaggtgacc ccgcagcctc aaaccccagt tcagcccccg
     2821 tctgtggcta cccctcagtc atcgcagcaa cagccgacgc ctgtgcacgc ccagcctcct
     2881 ggcacaccgc tttcccaggc agcagccagc attgataaca gagtccctac cccctcctcg
     2941 gtggccagcg cagaaaccaa ttcccagcag ccaggacctg acgtacctgt gctggaaatg
     3001 aagacggaga cccaagcaga ggacactgag cccgatcctg gtgaatccaa aggggagccc
     3061 aggtctgaga tgatggagga ggatttgcaa ggagcttccc aagttaaaga agaaacagac
     3121 atagcagagc agaaatcaga accaatggaa gtggatgaaa agaaacctga agtgaaagta
     3181 gaagttaaag aggaagaaga gagtagcagt aacggcacag cctctcagtc aacatctcct
     3241 tcgcagccgc gcaaaaaaat ctttaaacca gaggagttac gccaggccct catgccaacc
     3301 ctagaagcac tgtatcgaca ggacccagag tcattacctt tccggcagcc tgtagatccc
     3361 cagctcctcg gaattccaga ctattttgac atcgtaaaga atcccatgga cctctccacc
     3421 atcaagcgga agctggacac agggcaatac caagagccct ggcagtacgt ggacgacgtc
     3481 tggctcatgt tcaacaatgc ctggctctat aatcgcaaga catcccgagt ctataagttt
     3541 tgcagtaagc ttgcagaggt ctttgagcag gaaattgacc ctgtcatgca gtcccttgga
     3601 tattgctgtg gacgcaagta tgagttttcc ccacagactt tgtgctgcta tgggaagcag
     3661 ctgtgtacca ttcctcgcga tgctgcctac tacagctatc agaataggta tcatttctgt
     3721 gagaagtgtt tcacagagat ccagggcgag aatgtgaccc tgggtgacga cccttcacag
     3781 ccccagacga caatttcaaa ggatcagttt gaaaagaaga aaaatgatac cttagacccc
     3841 gaacctttcg ttgattgcaa ggagtgtggc cggaagatgc atcagatttg cgttctgcac
     3901 tatgacatca tttggccttc aggttttgtg tgcgacaact gcttgaagaa aactggcaga
     3961 cctcgaaaag aaaacaaatt cagtgctaag aggctgcaga ccacaagact gggaaaccac
     4021 ttggaagacc gagtgaacaa atttttgcgg cgccagaatc accctgaagc cggggaggtt
     4081 tttgtccgag tggtggccag ctcagacaag acggtggagg tcaagcccgg gatgaagtca
     4141 cggtttgtgg attctgggga aatgtctgaa tctttcccat atcgaaccaa agctctgttt
     4201 gcttttgagg aaattgacgg cgtggatgtc tgcttttttg gaatgcacgt ccaagaatac
     4261 ggctctgatt gcccccctcc aaacacgagg cgtgtgtaca tttcttatct ggatagtatt
     4321 catttcttcc ggccacgttg cctccgcaca gccgtttacc atgagatcct tattggatat
     4381 ttagagtatg tgaagaaatt agggtatgtg acagggcaca tctgggcctg tcctccaagt
     4441 gaaggagatg attacatctt ccattgccac ccacctgatc aaaaaatacc caagccaaaa
     4501 cgactgcagg agtggtacaa aaagatgctg gacaaggcgt ttgcagagcg gatcatccat
     4561 gactacaagg atattttcaa acaagcaact gaagacaggc tcaccagtgc caaggaactg
     4621 ccctattttg aaggtgattt ctggcccaat gtgttagaag agagcattaa ggaactagaa
     4681 caagaagaag aggagaggaa aaaggaagag agcactgcag ccagtgaaac cactgagggc
     4741 agtcagggcg acagcaagaa tgccaagaag aagaacaaca agaaaaccaa caagaacaaa
     4801 agcagcatca gccgcgccaa caagaagaag cccagcatgc ccaacgtgtc caatgacctg
     4861 tcccagaagc tgtatgccac catggagaag cacaaggagg tcttcttcgt gatccacctg
     4921 cacgctgggc ctgtcatcaa caccctgccc cccatcgtcg accccgaccc cctgctcagc
     4981 tgtgacctca tggatgggcg cgacgccttc ctcaccctcg ccagagacaa gcactgggag
     5041 ttctcctcct tgcgccgctc caagtggtcc acgctctgca tgctggtgga gctgcacacc
     5101 cagggccagg accgctttgt ctacacctgc aacgagtgca agcaccacgt ggagacgcgc
     5161 tggcactgca ctgtgtgcga ggactacgac ctctgcatca actgctataa cacgaagagc
     5221 catgcccata agatggtgaa gtgggggctg ggcctggatg acgagggcag cagccagggc
     5281 gagccacagt caaagagccc ccaggagtca cgccggctga gcatccagcg ctgcatccag
     5341 tcgctggtgc acgcgtgcca gtgccgcaac gccaactgct cgctgccatc ctgccagaag
     5401 atgaagcggg tggtgcagca caccaagggc tgcaaacgca agaccaacgg gggctgcccg
     5461 gtgtgcaagc agctcatcgc cctctgctgc taccacgcca agcactgcca agaaaacaaa
     5521 tgccccgtgc ccttctgcct caacatcaaa cacaagctcc gccagcagca gatccagcac
     5581 cgcctgcagc aggcccagct catgcgccgg cggatggcca ccatgaacac ccgcaacgtg
     5641 cctcagcaga gtctgccttc tcctacctca gcaccgcccg ggacccccac acagcagccc
     5701 agcacacccc agacgccgca gccccctgcc cagccccaac cctcacccgt gagcatgtca
     5761 ccagctggct tccccagcgt ggcccggact cagcccccca ccacggtgtc cacagggaag
     5821 cctaccagcc aggtgccggc ccccccaccc ccggcccagc cccctcctgc agcggtggaa
     5881 gcggctcggc agatcgagcg tgaggcccag cagcagcagc acctgtaccg ggtgaacatc
     5941 aacaacagca tgcccccagg acgcacgggc atggggaccc cggggagcca gatggccccc
     6001 gtgagcctga atgtgccccg acccaaccag gtgagcgggc ccgtcatgcc cagcatgcct
     6061 cccgggcagt ggcagcaggc gccccttccc cagcagcagc ccatgccagg cttgcccagg
     6121 cctgtgatat ccatgcaggc ccaggcggcc gtggctgggc cccggatgcc cagcgtgcag
     6181 ccacccagga gcatctcacc cagcgctctg caagacctgc tgcggaccct gaagtcgccc
     6241 agctcccctc agcagcaaca gcaggtgctg aacattctca aatcaaaccc gcagctaatg
     6301 gcagctttca tcaaacagcg cacagccaag tacgtggcca atcagcccgg catgcagccc
     6361 cagcctggcc tccagtccca gcccggcatg caaccccagc ctggcatgca ccagcagccc
     6421 agcctgcaga acctgaatgc catgcaggct ggcgtgccgc ggcccggtgt gcctccacag
     6481 cagcaggcga tgggaggcct gaacccccag ggccaggcct tgaacatcat gaacccagga
     6541 cacaacccca acatggcgag tatgaatcca cagtaccgag aaatgttacg gaggcagctg
     6601 ctgcagcagc agcagcaaca gcagcagcaa caacagcagc aacagcagca gcagcaaggg
     6661 agtgccggca tggctggggg catggcgggg cacggccagt tccagcagcc tcaaggaccc
     6721 ggaggctacc caccggccat gcagcagcag cagcgcatgc agcagcatct ccccctccag
     6781 ggcagctcca tgggccagat ggcggctcag atgggacagc ttggccagat ggggcagccg
     6841 gggctggggg cagacagcac ccccaacatc cagcaagccc tgcagcagcg gattctgcag
     6901 caacagcaga tgaagcagca gattgggtcc ccaggccagc cgaaccccat gagcccccag
     6961 caacacatgc tctcaggaca gccacaggcc tcgcatctcc ctggccagca gatcgccacg
     7021 tcccttagta accaggtgcg gtctccagcc cctgtccagt ctccacggcc ccagtcccag
     7081 cctccacatt ccagcccgtc accacggata cagccccagc cttcgccaca ccacgtctca
     7141 ccccagactg gttcccccca ccccggactc gcagtcacca tggccagctc catagatcag
     7201 ggacacttgg ggaaccccga acagagtgca atgctccccc agctgaacac ccccagcagg
     7261 agtgcgctgt ccagcgaact gtccctggtc ggggacacca cgggggacac gctagagaag
     7321 tttgtggagg gcttgtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     7381 ctttatatat cttgtggaaa ggacgaatcc gaccactgac tatcgagtcc
//
