LOCUS       sequence-281906-        2012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1175
FEATURES             Location/Qualifiers
     source          1..2012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcccattt gatgatgttc agggatgtgg ctgtagattt ctctcaggag
       61 gagtgggagt gcctggacct ggaacagagg gatttatata gagatgtgat gttggagaac
      121 tacagcaaca tggtctcact gggtttttgc atttatcagc cagaagcgtt ctctttattg
      181 gagaaaggga aagagccctg gaagattttg agggatgaga caagaggacc ttgcccagac
      241 atgcagtcga ggtgtcagac caagaagtta ttaccaaaaa atggcatttt tgagagagaa
      301 atagcccaat tggaaataat gagaatttgt aaaaaccaca gccttgactg tttatgtttt
      361 agaggtgact gggaaggcaa cactcagttt caaacacttc aagataatca agaggaatgc
      421 ttcaagcagg tgatacgcac ctgtgaaaaa aggcccactt ttaaccagca tacagtgttt
      481 aatctacacc agagacttaa tacaggagac aaactgaatg aatttaaaga actggggaaa
      541 gcctttattt ctggttcaga tcatactcaa catcagttaa ttcacacaag tgagaaattc
      601 tgtggagata aagaatgtgg gaataccttt cttcctgatt cagaagttat tcaatatcag
      661 acagttcaca ctgttaagaa aacatatgaa tgtaaagaat gtgggaagtc ttttagttta
      721 cgttcgagtc ttactggtca taagagaatt cataccggtg agaaaccttt taaatgtaag
      781 gattgtggga aagcctttag atttcattca caacttagtg tccataagcg aattcatact
      841 ggtgagaaat cttatgaatg taaggaatgt gggaaggcct ttagttgtgg ctcagatctt
      901 actcgacatc agagaattca tactggtgaa aaaccctatg aatgtaatga atgtagaaag
      961 gcctttagtc agcgatcaca tcttattaaa catcagagaa ttcacactgg tgaaaaacct
     1021 tatgaatgta aggagtgtgg gaaagctttt actcgtggat cacacctaac tcagcatcag
     1081 agaattcata ctggtgagaa atctcatgag tgtaaggaat gtggaaaagc ctttattcgt
     1141 ggttcaaatc ttgctcaaca tcagaatgtt catgttggta ggaaacctta taaatgtgag
     1201 aaatgtggga aagcctatat ttggagctca caccttgctc gacatcagcg aattcatact
     1261 ggcaggaaac cttatgaatg taagcaatgc gggaagactt ttacttgggc ttcgtatctt
     1321 gctcaacatg agaaaattca caatgagagg aaatcctatg aatgtaagga atgtggaaag
     1381 acctttcttc atggctcaga gtttaatcga catcagaaaa ttcatactgg tgagagaaac
     1441 tatgaatgta aggaatgtgg aaagaccttt tttcgtggtt cagaacttaa tcgacaccag
     1501 aaaattcata ctggaaagag gccatatgaa tgtgaagaat gtggaaaagc ctttctctgg
     1561 ggttcacaac ttactcgaca tcagagaatg catactggtg aggaacctta cgtatgtaaa
     1621 gaatgtggga aatcttttat ctggggttca cagcttacac gacataagaa aattcatact
     1681 gatgcagaac cttatggatg caagaaaagt agccacatct ttagtcacca ttcatatttt
     1741 actgaacaaa aaattcataa tagtgcaaat ctctgtgaat ggacagacta tgggaacacc
     1801 tttagtcatg agtcaaactt tgctcaacac cagaatattt acacttttga gaaatcctat
     1861 gaatttaaag attttgagaa agcattttct tcaagctctc acttcatttc actcttgtag
     1921 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1981 aaggacgact tgtcccgacg taaacgacgg gc
//
