LOCUS       sequence-281912-        2819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1309
FEATURES             Location/Qualifiers
     source          1..2819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaaggaga atcaagcaga tttgaaatcc acactccagt ttctgacaag
       61 aaaaagaaaa agtgttctat acataaggaa agacctcaga aacattccca cgaaattttc
      121 agagactcct ccctggtgaa tgaacagtct caaataacta ggaggaaaaa gaggaaaaaa
      181 gatttccagc atctcatttc ttctcctttg aaaaaatcca gaatctgtga tgagactgca
      241 aatgccactt ccacactcaa aaagagaaaa aagagaagat atagtgcttt ggaggtggac
      301 gaggaagcag gtgttacagt tgtccttgtg gataaagaaa atattaacaa cacaccaaag
      361 cattttagaa aggatgttga tgttgtttgt gttgatatga gcatagaaca gaagttacca
      421 agaaagccta aaacagacaa atttcaggta cttgctaagt cacatgcaca taaatcagaa
      481 gccctgcaca gtaaagttag ggagaaaaag aataaaaagc atcagaggaa agctgcatcc
      541 tgggagagcc agcgggcaag ggacaccctg cctcagtcag aatcccacca ggaggagtcc
      601 tggctttctg tgggtccagg gggtgaaatt acagaactac cagcatctgc tcataaaaac
      661 aagtctaaga aaaaaaagaa aaagtccagt aaccgggaat atgagacact ggccatgcct
      721 gaaggatcgc aagcaggcag agaggccggg actgatatgc aggaatccca gcctactgtg
      781 ggcttggatg atgaaactcc acaactacta ggacctactc acaaaaaaaa gtctaagaaa
      841 aaaaagaaga aaaagtccaa tcaccaggaa tttgaggcat tggccatgcc tgaaggatca
      901 caagtgggca gtgaggttgg ggctgatatg caggaatccc ggcctgctgt gggcctgcat
      961 ggtgaaactg caggaatacc agcacctgct tataaaaaca agtctaagaa aaaaaagaaa
     1021 aagtccaatc accaggaatt tgaggcagtg gccatgcctg agagcctcga gagtgcatac
     1081 cctgaaggat cacaggtggg cagtgaggtt gggactgtgg aaggcagtac agctcttaaa
     1141 gggttcaagg aatccaacag tacaaagaag aagtctaaga aaaggaagct tacgtctgtc
     1201 aaaagggcac gagtgtctgg tgatgatttt tcagtgccca gtaagaactc tgagagcaca
     1261 ctctttgatt cagtagaagg tgatggcgcc atgatggaag aaggtgtgaa atctaggccc
     1321 cgacaaaaga aaacccaggc ctgtttggca agcaagcacg tgcaagaggc gccaaggtta
     1381 gaacctgcaa atgaagaaca caatgtggaa acagctgaag attccgaaat aagatactta
     1441 tctgcagatt caggagatgc cgatgattca gatgcggatt tgggttctgc cgtgaaacag
     1501 cttcaggagt tcattcctaa catcaaggac agggccacca gcacaatcaa gcggatgtac
     1561 cgggacgact tggaacggtt taaggaattt aaagcacaag gtgtcgctat taaatttggc
     1621 aagttttctg taaaggaaaa taagcagtta gagaaaaatg tggaagactt tctagccctg
     1681 acaggcattg agagtgcaga caagctgctg tacacggaca gatatcctga ggaaaaatct
     1741 gtgatcacca acttaaaaag gagatactcg tttagattac acattggtag gaacattgcc
     1801 cggccctgga aacttatata ctatcgagca aagaagatgt tcgatgtcaa caattacaaa
     1861 ggcaggtata gcgaaggaga tactgagaag ttaaagatgt accattctct ccttgggaat
     1921 gactggaaga cgattggtga gatggtggcc cgaagtagcc tctccgtggc cctcaagttc
     1981 tcacagatca gcagtcaaag aaatcgtggt gcttggagta agtctgaaac ccggaaacta
     2041 atcaaggctg tcgaagaagt gattctgaag aagatgtctc cccaggagtt aaaagaggtg
     2101 gattccaaac tccaagaaaa tcctgaaagt tgcctatcaa ttgttcggga aaaactctac
     2161 aagggcatat cttgggtaga agtagaagct aaagtgcaaa ccagaaattg gatgcagtgt
     2221 aaaagtaagt ggacagaaat tctaaccaag aggatgacta atggtcggcg tatctactat
     2281 ggcatgaatg ccctgcgggc caaggtcagc cttattgaaa ggttgtatga aataaatgtg
     2341 gaagatacta atgaaataga ctgggaagat cttgctagtg ccataggtga tgttcctcca
     2401 tcttacgttc aaactaaatt ttctaggctg aaagctgtct atgttccatt ttggcagaaa
     2461 aagacttttc cagagatcat cgactacctt tatgagacga ctctaccttt gctgaaggaa
     2521 aagttagaaa aaatgatgga gaaaaaaggc actaaaatcc agactcctgc agcacccaag
     2581 caagttttcc catttcgaga catcttttat tatgaagacg atagtgaagg agaggacata
     2641 gaaaaagaaa gcgaaggcca ggcgccatgc atggctcacg cctgtaattc cagtactttg
     2701 ggaggccaag gccggtggat catctagtaa tgaactagtc cgtaacttga aagtatttcg
     2761 atttcttggc tttatatatc ttgtggaaag gacgaggtga tacttacaca catcgggtt
//
