LOCUS       sequence-281915-        1655 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1314
FEATURES             Location/Qualifiers
     source          1..1655
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccgccgc tatcaccgac atggccgacc tggaggagct ctcccgcctg
       61 agccctctgc cccccggcag cccgggttcg gcggcgcggg gccgggctga gccccccgag
      121 gaggaggagg aagaggagga ggaggaagag gaggcggagg ccgaggcggt ggcggcgctg
      181 ctgctgaacg gcggcagcgg tgggggcggc ggaggcggcg gcggaggagt ggggggcggc
      241 gaggcagaga cgatgtcgga gccgagcccc gagagcgcca gccaggccgg ggaggacgaa
      301 gacgaggagg aggacgacga ggaggaggaa gatgagagca gcagcagcgg cgggggtgag
      361 gaggagagta gcgccgagag cctggtgggc agcagcggcg ggagcagcag cgacgagacc
      421 cgctcgttga gccccggcgc cgccagcagc agcagcgggg atggggacgg caaggagggc
      481 ctggaggagc ccaagggacc gcggggcagc cagggcggcg gcgggggcgg cagcagtagc
      541 agcagcgtag tctccagcgg cggcgacgag ggctacggga ctgggggagg cggaagcagc
      601 gcgacctccg ggggccggcg gggcagcttg gagatgtcgt cggatgggga acccctgagc
      661 cgcatggact cggaggacag cataagcagt actataatgg atgtagacag cacaatttcc
      721 agtgggcgtt caactccagc aatgatgaat ggacaaggaa gcactacttc ttcaagcaaa
      781 aatattgcct ataattgttg ttgggaccag tgccaggctt gcttcaactc tagcccagat
      841 ctggcagatc acatccgttc catacatgta gatggtcagc gaggaggggt atttgtttgc
      901 ttatggaaag gttgtaaagt atataacact ccatctacca gtcaaagttg gttacaaagg
      961 catatgctga cacacagtgg agacaaacct ttcaagtgtg ttgttggtgg ctgcaatgcc
     1021 agctttgctt ctcagggagg gctagctcgt catgtaccca cacacttcag tcagcagaac
     1081 tcctcaaaag tttctagcca gccaaaggcc aaagaagaat ctccttctaa agctggaatg
     1141 aacaaaagga ggaaattaaa gaacaaaaga cgacgctcat taccacggcc acatgatttc
     1201 ttcgatgcac aaacactgga tgcgataaga catcgagcca tatgctttaa cctctcagct
     1261 catatagaaa gtttagggaa gggacacagt gttgtttttc atagtactgt aatagctaag
     1321 agaaaagaag attctgggaa gatcaaactt ttgcttcatt ggatgcctga agacattctg
     1381 cctgatgtgt gggtgaatga aagtgaacga catcagttaa aaactaaagt agttcattta
     1441 tcaaagctac ccaaagatac tgccttgctt ttggacccaa acatatacag aacaatgccg
     1501 cagaagaggt tgaagagaac tctgataaga aaagtgttca atttgtattt aagcaaacag
     1561 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     1621 ggaaaggacg acgtttccga tccgaatggc ggcta
//
