LOCUS       sequence-281918-        1955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1315
FEATURES             Location/Qualifiers
     source          1..1955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctcttcc tcagggtcta ttgacattca gggatgtggc catagaattc
       61 tctcaggagg agtggaaatg cctggaccct gctcagagga ctctatacag ggacgtgatg
      121 ctggagaatt ataggaacct ggtctccctg gatatctctt ccaaatgcat gatgaaggag
      181 ttctcatcaa cagcacaagg caatagagaa gtgatccaca cagggacatt gcaaagacat
      241 gaaagtcatc acactggaga ctttcgcttt caggaaattg ataaagatat tcataactta
      301 gagtttcagt ggcaagaaga tgaaagaaat agccatgaag cacccatgac agaaatcaaa
      361 aagttgactg gtagtgcaga ccgatatgat caaaggcatg ctggaaacaa gcctattaaa
      421 gatcagcttg gatcaagctt tcattcgcat ctgcctgaac tccacatgtt tcagacccaa
      481 gggaaaattg gtaatcaagt ggagaagtct atcaacgatg cttcctcaat ttcaacatcc
      541 caaagaattt cttgtaggcc caaaacccat atttctaata actatgggaa taatttccgg
      601 aattcttcgt tactcacaca aaaacaggag gtacacatga gagaaaagtc tttccaatgt
      661 aatgagagtg gcaaagcctt taattatagc tcactcttaa ggaaacatca aataatccat
      721 ttaggagaga aacaatataa atgtgatgta tgtggcaagg tctttaatcg gaagcgaaac
      781 ctagtgtgcc atcgtagatg tcacactggg gagaaacctt acaggtgtaa tgagtgtggc
      841 aagactttca gtcagacgta ttcccttaca tgccatcgta gacttcatac tggagagaaa
      901 ccttacaaat gtgaagaatg tgacaaagct ttcagtttca aatcaaacct taaaagacat
      961 aggagaattc atgctggaga aaaaccatac aagtgtaatg aatgtggcaa gacctttagt
     1021 cagacgtcat cccttacatg ccatcgtaga cttcatactg gagagaaacc tttcaagtgt
     1081 aatgagtgtg gcaagacctt tagtcggaag tcatccctta catgccatca tagacttcat
     1141 acgggagaga aaccttataa gtgtaatgaa tgtggcaaga ccttcagtca ggagttaacc
     1201 cttaaatgcc atcgtagact tcataccgga gagaagcctt acaagtgtaa tgaatgtggc
     1261 aaggttttta ataaaaaggc aaaccttgca cgtcatcata gacttcatag tggagagaaa
     1321 ccctacaagt gtactgagtg tgtcaagacg ttcagtcgaa attcagccct tgtaattcat
     1381 aaggctattc atattggaga gaaacgttac aagtgtaatg agtgtggcaa gacgttcagt
     1441 cgaatttcag ccctcgtaat tcatacggca attcatactg gagagaaacc ttacaagtgt
     1501 aatgaatgtg gcaagggttt taatcggaaa acacaccttg catgtcatca tagacttcat
     1561 actggagaga aaccttacaa gtgtaatgaa tgtggcaagg tttttaatcg aaaaacacac
     1621 cttgcacatc atcatagact tcatactgga gataaacctt acaagtgtaa tgaatgtggc
     1681 aaggttttta atcaaaaagc acaccttgca cgtcaccata gacttcatac tggagagaaa
     1741 ccttacaagt gtaatgaatg tggcaaggtt tttaatcaaa aagcaaacct tgcacgtcat
     1801 catagacttc atactggaga gaaaccttac aagtttaatg agtgtggcaa agcttttaat
     1861 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     1921 ggaaaggacg atttcatatt actaggatca tgact
//
