LOCUS       sequence-281945-        1922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1417
FEATURES             Location/Qualifiers
     source          1..1922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagtaac atttgaagat gtgactatta tttttacatg ggaggagtgg
       61 aaattcctgg attcttctca aaaaagactc tacagggagg tcatgtggga gaactacaca
      121 aatgtcatgt cagtagaaaa ctggaatgag agctacaaat cccaagaaga aaaattcaga
      181 tacttagaat atgaaaattt ttcctactgg caaggctggt ggaatgctgg cgcccagatg
      241 tatgagaatc agaactatgg ggaaactgtt caagggacag attccaaaga cctcacacag
      301 caagatcgtt cccagtgtca ggaatggtta atactctcca cacaagtacc agggtatggg
      361 aactatgaac tgacttttga aagcaaaagt ctcaggaact taaaatataa aaattttatg
      421 ccttggcagt ccttagaaac aaaaaccact caagactatg gtagagaaat ctacatgagt
      481 ggttcacatg gttttcaagg gggcagatac cgtcttggca tatccaggaa aaacctctcc
      541 atggaaaaag aacagaagct catagttcag cattcttata tcccagtgga ggaagccctt
      601 ccacagtatg ttggggtgat atgtcaagaa gacctactga gagattcaat ggaagaaaag
      661 tactgtggat gtaataaatg taaaggaatt tattattgga actcacggtg tgttttccac
      721 aagagaaatc aacctggaga aaacctctgt caatgctcca tctgtaaagc atgcttctct
      781 cagagatcag acttgtatag acatccaaga aaccacatag gtaagaagct gtacggatgt
      841 gatgaagttg acggtaactt tcatcagagc tccggagttc actttcatca gagagttcac
      901 ataggggagg taccttatag ctgtaatgca tgtggtaaga gcttcagcca gatctctagt
      961 cttcacaatc atcaaagagt ccacacagaa gagaaattct ataaaattga gtgtgataaa
     1021 gacctcagta gaaattcatt acttcacatt caccagagac ttcacatagg agagaagcct
     1081 tttaaatgta atcagtgtgg taagagtttt aatcggagtt cagtacttca tgttcatcag
     1141 agagtccaca caggagaaaa accatataag tgtgatgagt gtggtaaggg tttcagccag
     1201 agctcaaatc ttcgaattca tcagttagta cacacaggag agaagtctta taaatgtgaa
     1261 gactgtggta aaggctttac ccagcgctca aatcttcaaa ttcatcagag agtgcataca
     1321 ggagagaaac cttataaatg tgatgactgt ggaaaggact ttagtcacag ctcagatctt
     1381 cgcattcatc agagagtcca tacaggggag aaaccctata cttgtcctga atgtgggaag
     1441 ggcttcagta agagttcaaa gcttcacact catcaaagag tacatactgg agagaaaccc
     1501 tacaaatgtg aagagtgtgg caagggattc agtcagcgtt cacatcttct cattcatcag
     1561 agagtccata caggagaaaa gccctataaa tgtcatgatt gtggaaaggg ttttagtcac
     1621 agttctaatc ttcacattca tcagagggtc catacaggag agaagcctta tcaatgtgct
     1681 aagtgtggta aaggtttcag tcatagctca gctcttcgaa ttcatcaaag agtccatgca
     1741 ggagagaaac cttacaaatg ccgtgaatat tataagggat ttgatcataa ttcacatctt
     1801 cacaataatc atagaagagg aaacttatag taatgaacta gtccgtaact tgaaagtatt
     1861 tcgatttctt ggctttatat atcttgtgga aaggacgacg gctcgatgct cgccacagga
     1921 gg
//
