LOCUS       sequence-281948-        2084 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1426
FEATURES             Location/Qualifiers
     source          1..2084
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggcggc cgcggcggcg ctctcgggcg cgggcacgcc acccgcgggc
       61 ggcggggccg ggggcggcgg ggccgggggc ggcgggtccc cgccgggcgg ctgggccgtg
      121 gcgcgcctgg agggccgcga gttcgagtat ctgatgaaga agcgctcggt gaccatcggc
      181 cgcaactcgt cgcagggctc ggtggacgtg agcatgggcc actcgagctt catctcccgg
      241 cgccacctcg agatcttcac gcccccgggc ggcggcggcc atggcggggc cgctccggag
      301 ctgccgcccg cgcagcccag gcccgacgcc ggcggcgact tctacctgcg ctgcttgggc
      361 aagaacgggg tattcgtgga cggcgtgttc cagaggcgcg gggcgccgcc gctgcagctg
      421 ccgcgcgtgt gcacattcag gttcccgagc acaaacatca agataacgtt cactgccctg
      481 tccagcgaga agagagagaa gcaggaggcg tctgagtctc cagtgaaggc cgtacagcca
      541 cacatctcgc ccctgaccat caacattcca gacaccatgg cccacctcat cagccctctg
      601 ccctccccca cgggaaccat cagcgctgca aactcctgcc cctccagccc ccggggagcg
      661 gggtcttcag ggtacaaggt gggccgagtg atgccatctg acctcaattt aatggctgac
      721 aactcacagc ctgaaaatga aaaggaagct tcaggtggag acagcccgaa ggatgattca
      781 aagccgcctt actcctacgc gcagctgata gttcaggcga ttacgatggc tcccgacaaa
      841 cagctcaccc tgaacgggat ttatacacac atcactaaaa attatcccta ctacaggact
      901 gcggacaagg gctggcagaa ttcaattcgc cacaatctct ctctgaatcg ttatttcatc
      961 aaagtgccgc gttcccagga agaaccaggc aaaggctcgt tctggaggat agacccagcc
     1021 tctgaaagca aattaataga acaggctttt aggaaacgac ggcctagggg cgtgccctgc
     1081 tttagaaccc ctctgggacc gctctcttct aggagtgccc cagcctctcc caatcacgcg
     1141 ggagtgctgt ctgctcactc tagtggcgcc cagacccctg agagcctgtc gagggaaggt
     1201 tcgccggccc ccctggagcc tgagcctggc gctgcacagc ccaaactcgc tgtcatccag
     1261 gaagcccggt ttgcccagag cgccccaggg tcacctctgt ccagtcagcc agtcttaatc
     1321 accgtccagc ggcagctacc acaggccatc aagcctgtca cctacactgt ggccacccca
     1381 gtgaccacct cgacctccca gccacccgtc gtgcagacgg ttcacgtcgt ccaccagatc
     1441 ccagcggtgt cggtcaccag tgtggccgga ctggccccag cgaacacgta cactgtctct
     1501 ggacaagctg tggtcacccc ggcagccgtg ctggcccctc ctaaggcaga ggcccaggag
     1561 aatggagacc acagggaagt caaagtgaaa gtagagccta ttcccgccat tggccacgcc
     1621 acgctcggca ctgccagccg gatcattcag acggcacaga ccaccccggt ccagacggtg
     1681 accatagtac aacaggcacc tctaggtcaa caccagctac caataaaaac tgtaacacaa
     1741 aacggcactc acgtggcatc agtccccact gcggtccacg gccaggtgaa caatgccgcg
     1801 gcgagtcctt tgcacatgtt ggcaacacac gcatccgcat cggcctccct gcccacaaag
     1861 cgccacaacg gtgaccagcc ggagcagccg gagctgaagc ggatcaagac agaagacggc
     1921 gagggcatcg tcattgccct gagcgtggac acgccaccgg cagccgtaag ggaaaagggt
     1981 gtccagaact agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     2041 atatcttgtg gaaaggacga gtcaacagat tcgctacccg acga
//
