LOCUS       sequence-281972-        1511 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1447
FEATURES             Location/Qualifiers
     source          1..1511
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggtgaatta ctcctatgat gaagatctgg aagagctttg tcccgtgtgt
       61 ggagataaag tgtctgggta ccattatggg ctcctcacct gtgaaagctg caagggattt
      121 tttaagcgaa cagtccaaaa taataaaagg tacacatgta tagaaaacca gaactgccaa
      181 attgacaaaa cacagagaaa gcgttgtcct tactgtcgtt ttcaaaaatg tctaagtgtt
      241 ggaatgaagc tagaagctgt aagggccgac cgaatgcgtg gaggaaggaa taagtttggg
      301 ccaatgtaca agagagacag ggccctgaag caacagaaaa aagccctcat ccgagccaat
      361 ggacttaagc tagaagccat gtctcaggtg atccaagcta tgccctctga cctgaccatt
      421 tcctctgcaa ttcaaaacat ccactctgcc tccaaaggcc tacctctgaa ccatgctgcc
      481 ttgcctccta cagactatga cagaagtccc tttgtaacat cccccattag catgacaatg
      541 ccccctcacg gcagcctgca aggttaccaa acatatggcc actttcctag ccgggccatc
      601 aagtctgagt acccagaccc ctataccagc tcacccgagt ccataatggg ctattcatat
      661 atggatagtt accagacgag ctctccagca agcatcccac atctgatact ggaacttttg
      721 aagtgtgagc cagatgagcc tcaagtccag gctaaaatca tggcctattt gcagcaagag
      781 caggctaacc gaagcaagca cgaaaagctg agcacctttg ggcttatgtg caaaatggca
      841 gatcaaactc tcttctccat tgtcgagtgg gccaggagta gtatcttctt cagagaactt
      901 aaggttgatg accaaatgaa gctgcttcag aactgctgga gtgagctctt aatcctcgac
      961 cacatttacc gacaagtggt acatggaaag gaaggatcca tcttcctggt tactgggcaa
     1021 caagtggact attccataat agcatcacaa gccggagcca ccctcaacaa cctcatgagt
     1081 catgcacagg agttagtggc aaaacttcgt tctctccagt ttgatcaacg agagttcgta
     1141 tgtctgaaat tcttggtgct ctttagttta gatgtcaaaa accttgaaaa cttccagctg
     1201 gtagaaggtg tccaggaaca agtcaatgcc gccctgctgg actacacaat gtgtaactac
     1261 ccgcagcaga cagagaaatt tggacagcta cttcttcgac tacccgaaat ccgggccatc
     1321 agtatgcagg ctgaagaata cctctactac aagcacctga acggggatgt gccctataat
     1381 aaccttctca ttgaaatgtt gcatgccaaa agagcatagt aatgaactag tccgtaactt
     1441 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgacaa tcaaggcggt
     1501 cgcctcgttt g
//
