LOCUS       sequence-281975-        1088 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1453
FEATURES             Location/Qualifiers
     source          1..1088
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             934..954
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
ORIGIN
        1 accgccacca tgactggagt ctttgacagt ctagtggctg atatgcactc gacccagatc
       61 gccgcctcca gcacgtacca ccagcaccag cagcccccga gcggcggcgg cgccggcccg
      121 ggtggcaaca gcagcagcag cagcagcctc cacaagcccc aggagtcgcc cacccttccg
      181 gtgtccaccg ccaccgacag cagctactac accaaccagc agcacccggc gggcggcggc
      241 ggcggcgggg gctcgcccta cgcgcacatg ggttcctacc agtaccaagc cagcggcctc
      301 aacaacgtcc cttactccgc caagagcagc tatgacctgg gctacaccgc cgcctacacc
      361 tcctacgctc cctatggaac cagttcgtcc ccagccaaca acgagcctga gaaggaggac
      421 cttgagcctg aaattcggat agtgaacggg aagccaaaga aagtccggaa accccgcacc
      481 atctactcca gtttccagct ggcggctctt cagcggcgtt tccaaaagac tcaatacttg
      541 gccttgccgg agcgagccga gctggcggcc tctctgggcc tcacccagac tcaggtcaaa
      601 atctggttcc agaaccgccg gtccaagttc aagaagatgt ggaaaagtgg tgagatcccc
      661 tcggagcagc accctggggc cagcgcttct ccaccttgtg cttcgccgcc agtctcagcg
      721 ccggcctcct gggactttgg tgtgccgcag cggatggcgg gcggcggtgg tccgggcagt
      781 ggcggcagcg gcgccggcag ctcgggctcc agcccgagca gcgcggcctc ggcttttctg
      841 ggcaactacc cctggtacca ccagacctcg ggatccgcct cacacctgca ggccacggcg
      901 ccgctgctgc accccactca gaccccgcag ccgcatcacc accaccacca tcacggcggc
      961 gggggcgccc cggtgagcgc ggggacgatt ttctagtaat gaactagtcc gtaacttgaa
     1021 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaccttga taaggaaata
     1081 gcggtggc
//
