LOCUS       sequence-281981-        1574 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1458
FEATURES             Location/Qualifiers
     source          1..1574
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      46..55
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgacagcaag ctcaggctgg gagccagcgc cggcagccac catggcgtca
       61 cgcatagggt tgcgcatgca gctcatgcgg gagcaggcgc agcaggagga gcagcgggag
      121 cgcatgcagc aacaggctgt catgcattac atgcagcagc agcagcagca gcaacagcag
      181 cagctcggag ggccgcccac cccggccatc aatacccccg tccacttcca gtcgccacca
      241 cctgtgcctg gggaggtgtt gaaggtgcag tcctacctgg agaatcccac atcctaccat
      301 ctgcagcagt cgcagcatca gaaggtgcgg gagtacctgt ccgagaccta tgggaacaag
      361 tttgctgccc acatcagccc agcccagggc tctccgaaac ccccaccagc cgcctcccca
      421 ggggtgcgag ctggacacgt gctgtcctcc tccgctggca acagtgctcc caatagcccc
      481 atggccatgc tgcacattgg ctccaaccct gagagggagt tggatgatgt cattgacaac
      541 attatgcgtc tggacgatgt ccttggctac atcaatcctg aaatgcagat gcccaacacg
      601 ctacccctgt ccagcagcca cctgaatgtg tacagcagcg acccccaggt cacagcctcc
      661 ctggtgggcg tcaccagcag ctcctgccct gcggacctga cccagaagcg agagctcaca
      721 gatgctgaga gcagggccct ggccaaggag cggcagaaga aagacaatca caacttaatt
      781 gaaaggagac gaaggttcaa catcaatgac cgcatcaagg agttgggaat gctgatcccc
      841 aaggccaatg acctggacgt gcgctggaac aagggcacca tcctcaaggc ctctgtggat
      901 tacatccgga ggatgcagaa ggacctgcaa aagtccaggg agctggagaa ccactctcgc
      961 cgcctggaga tgaccaacaa gcagctctgg ctccgtatcc aggagctgga gatgcaggct
     1021 cgagtgcacg gcctccctac cacctccccg tccggcatga acatggctga gctggcccag
     1081 caggtggtga agcaggagct gcctagcgaa gagggcccag gggaggccct gatgctgggg
     1141 gctgaggtcc ctgaccctga gccactgcca gctctgcccc cgcaagcccc gctgcccctg
     1201 cccacccagc caccatcccc attccatcac ctggacttca gccacagcct gagctttggg
     1261 ggcagggagg acgagggtcc cccgggctac cccgaacccc tggcgccggg gcatggctcc
     1321 ccattcccca gcctgtccaa gaaggatctg gacctcatgc tcctggacga ctcactgcta
     1381 ccgctggcct ctgatccact tctgtccacc atgtcccccg aggcctccaa ggccagcagc
     1441 cgccggagca gcttcagcat ggaggagggc gatgtgctgt agtaatgaac tagtccgtaa
     1501 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga caggtcagga
     1561 ccttcctcct acat
//
