LOCUS       sequence-281990-        1532 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1488
FEATURES             Location/Qualifiers
     source          1..1532
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagcggga cgaaccgccg cctagcggag ggggaggcgg cgggggctcg
       61 gcggggttcc tggagcctcc cgccgcgctc cctccgccgc cgcgcaacgg tttctgtcag
      121 gatgaattgg cagagcttga cccaggcact aatgatcggg ctgaacaacg aacctgtctc
      181 atttgtgggg accgcgctac aggcttgcac tatgggatca tctcctgtga gggctgcaaa
      241 gggtttttca agcggagcat ttgcaacaaa cgggtatatc gatgcagtcg tgacaagaac
      301 tgtgtcatgt ctcggaagca gaggaacagg tgccagtact gccgcctgct caaatgcctc
      361 cagatgggga tgaaccggaa ggctatcaga gaagatggca tgcctggagg ccggaataag
      421 agcattgggc cagtccagat atcggaagaa gaaatcgaaa ggatcatgtc tgggcaggag
      481 tttgaggaag aggccaatca ctggagcaac catggtgata gtgaccacag ttcccctggg
      541 aacagggctt cggagagcaa ccagccctca ccaggctcca cactgtcttc cagtaggtct
      601 gtggaactga atggattcat ggccttcagg gaacagtaca tgggaatgtc tgtgcctcca
      661 cattaccaat atataccgca cctttttagc tattctggcc actcaccact tctgccccaa
      721 caagctcgca gcctggatcc ccagtcatac agtctgattc accagctgtt atcagccgag
      781 gacctggaac cattgggcac gcccatgttg attgaagatg gatacgctgt gacacaggca
      841 gaactatttg ccctgctttg ccgcctggcc gacgagctgc tctttaggca gattgcctgg
      901 atcaagaaac tgcctttctt ctgcgagctc tcaatcaagg attacacgtg cctcttgagc
      961 tctacgtggc aggagctaat cctgctgtct tccctcaccg tttacagcaa gcagatcttt
     1021 ggggaactgg ctgatgtcac tgccaagtac tcgccctccg atgaagaact acacagattt
     1081 agtgatgaag ggatggaggt gatcgagcgg ctcatctacc tctatcacaa gttccatcag
     1141 ctaaaggtca gcaacgagga gtatgcttgc atgaaagcaa ttaacttcct aaatcaagat
     1201 atcaggggtc tgaccagtgc ctcacagctg gaacaattga ataaacgata ctggtacatt
     1261 tgccaggatt ttactgaata taaatacaca catcagccga accgctttcc tgatctcatg
     1321 atgtgcttac ctgagattcg atatattgca ggaaagatgg tgaatgtgcc cctggagcag
     1381 ctgcccctcc tctttaaggt ggtgctgcat tcctgcaaga ccagtgtggg caaggaatag
     1441 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1501 aaggacgaac aggtatagca catgatgatg ta
//
