LOCUS       sequence-281993-        1544 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1489
FEATURES             Location/Qualifiers
     source          1..1544
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagcggga cgaaccgccg cctagcggag ggggaggcgg cgggggctcg
       61 gcggggttcc tggagcctcc cgccgcgctc cctccgccgc cgcgcaacgg tttctgtcag
      121 gatgaattgg cagagcttga cccaggcact atttctgttt cagatgatcg ggctgaacaa
      181 cgaacctgtc tcatttgtgg ggaccgcgct acaggcttgc actatgggat catctcctgt
      241 gagggctgca aagggttttt caagcggagc atttgcaaca aacgggtata tcgatgcagt
      301 cgtgacaaga actgtgtcat gtctcggaag cagaggaaca ggtgccagta ctgccgcctg
      361 ctcaaatgcc tccagatggg gatgaaccgg aaggctatca gagaagatgg catgcctgga
      421 ggccggaata agagcattgg gccagtccag atatcggaag aagaaatcga aaggatcatg
      481 tctgggcagg agtttgagga agaggccaat cactggagca accatggtga tagtgaccac
      541 agttcccctg ggaacagggc ttcggagagc aaccagccct caccaggctc cacactgtct
      601 tccagtaggt ctgtggaact gaatggattc atggccttca gggaacagta catgggaatg
      661 tctgtgcctc cacattacca atatataccg caccttttta gctattctgg ccactcacca
      721 cttctgcccc aacaagctcg cagcctggat ccccagtcat acagtctgat tcaccagctg
      781 ttatcagccg aggacctgga accattgggc acgcccatgt tgattgaaga tggatacgct
      841 gtgacacagg cagaactatt tgccctgctt tgccgcctgg ccgacgagct gctctttagg
      901 cagattgcct ggatcaagaa actgcctttc ttctgcgagc tctcaatcaa ggattacacg
      961 tgcctcttga gctctacgtg gcaggagcta atcctgctgt cttccctcac cgtttacagc
     1021 aagcagatct ttggggaact ggctgatgtc actgccaagt actcgccctc cgatgaagaa
     1081 ctacacagat ttagtgatga agggatggag gtgatcgagc ggctcatcta cctctatcac
     1141 aagttccatc agctaaaggt cagcaacgag gagtatgctt gcatgaaagc aattaacttc
     1201 ctaaatcaag atatcagggg tctgaccagt gcctcacagc tggaacaatt gaataaacga
     1261 tactggtaca tttgccagga ttttactgaa tataaataca cacatcagcc gaaccgcttt
     1321 cctgatctca tgatgtgctt acctgagatt cgatatattg caggaaagat ggtgaatgtg
     1381 cccctggagc agctgcccct cctctttaag gtggtgctgc attcctgcaa gaccagtgtg
     1441 ggcaaggaat agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1501 atatcttgtg gaaaggacga aaggtgaagt tgcgccaaac ggct
//
